β-catenin interacts with MyoD and regulates its transcription activity
β-catenin interacts with MyoD and regulates its transcription activity
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DOI：
10.1128/mcb.01682-07
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发表时间：
2008-05-01
影响因子：
5.3
通讯作者：
Mei, Lin
中科院分区：
文献类型：
--
作者：
Kim, Chang-Hoon;Neiswender, Hannah;Mei, Lin
Wnt对肌肉发育的调节被认为是由β-连环蛋白-TCF/LEF依赖的经典途径介导的。在这里，我们证明了β-连环蛋白，而不是TCF/LEF，是肌肉分化所必需的。我们发现β-连环蛋白直接与MyoD相互作用，MyoD是肌肉分化所必需的基本螺旋-环-螺旋转录因子，并增强其与E盒元件的结合和转录活性。当β-连环蛋白缺乏或MyoD和β-连环蛋白之间的相互作用被破坏时，MyoD介导的反式激活在肌细胞中被抑制。这些结果表明，β-连环蛋白是MyoD功能所必需的，将MyoD鉴定为Wnt经典途径中的效应子。
Wnt regulation of muscle development is thought to be mediated by the beta-catenin-TCF/LEF-dependent canonical pathway. Here we demonstrate that beta-catenin, not TCF/LEF, is required for muscle differentiation. We showed that beta-catenin interacts directly with MyoD, a basic helix-loop-helix transcription factor essential for muscle differentiation and enhances its binding to E box elements and transcriptional activity. MyoD-mediated transactivation is inhibited in muscle cells when beta-catenin is deficient or the interaction between MyoD and beta-catenin is disrupted. These results demonstrate that beta-catenin is necessary for MyoD function, identifying MyoD as an effector in the Wnt canonical pathway.