Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.
Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.
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高氮肥料下的比较磷酸蛋白质组学分析揭示了促进小麦籽粒淀粉和蛋白质合成的中心磷蛋白。
DOI：
10.3389/fpls.2017.00067
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发表时间：
2017
影响因子：
5.6
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Zhen S;Deng X;Zhang M;Zhu G;Lv D;Wang Y;Zhu D;Yan Y
氮（N）是植物生长发育的重要常量营养元素。它还强烈影响淀粉和蛋白质的合成，与谷物产量和品质密切相关。我们进行了第一次比较磷酸蛋白质组学分析的发展中小麦籽粒响应高氮肥。生理生化分析表明，高氮处理显著提高了小麦叶片长度和叶面积，叶绿素含量，叶片中硝酸还原酶（NR）活性和籽粒中蔗糖磷酸合成酶（SPS）、蔗糖合成酶（SuSy）、ADP葡萄糖焦磷酸化酶（AGG）活性。这种增强的酶活性导致淀粉含量、谷物产量和最终面包制作质量的显著改善。在开花后15和25天进行的高氮肥施用下的发育中的籽粒的比较磷酸蛋白质组学分析在1372个磷蛋白中鉴定了2470个磷酸化位点，其中411个独特的蛋白质显示磷酸化水平的显著变化（>2倍或<0.5倍）。这些磷蛋白主要参与信号转导、淀粉合成、能量代谢等。Pro-Q钻石染色和Western印迹证实了我们的磷酸化蛋白质组学结果。我们提出了一个假定的途径，以阐明中央磷蛋白调节谷物淀粉和蛋白质合成的重要作用。我们的研究结果提供了新的见解蛋白质磷酸化修饰的分子机制参与粮食的发展，产量和品质的形成。
Nitrogen (N) is a macronutrient important for plant growth and development. It also strongly influences starch and protein synthesis, closely related to grain yield and quality. We performed the first comparative phosphoproteomic analysis of developing wheat grains in response to high-N fertilizer. Physiological and biochemical analyses showed that application of high-N fertilizer resulted in significant increases in leaf length and area, chlorophyll content, the activity of key enzymes in leaves such as nitrate reductase (NR), and in grains such as sucrose phosphate synthase (SPS), sucrose synthase (SuSy), and ADP glucose pyrophosphorylase (AGPase). This enhanced enzyme activity led to significant improvements in starch content, grain yield, and ultimately, bread making quality. Comparative phosphoproteomic analysis of developing grains under the application of high-N fertilizer performed 15 and 25 days post-anthesis identified 2470 phosphosites among 1372 phosphoproteins, of which 411 unique proteins displayed significant changes in phosphorylation level (>2-fold or <0.5-fold). These phosphoproteins are involved mainly in signaling transduction, starch synthesis, energy metabolism. Pro-Q diamond staining and Western blotting confirmed our phosphoproteomic results. We propose a putative pathway to elucidate the important roles of the central phosphoproteins regulating grain starch and protein synthesis. Our results provide new insights into the molecular mechanisms of protein phosphorylation modifications involved in grain development, yield and quality formation.