Proanthocyanidin-Aluminum Complexes Improve Aluminum Resistance and Detoxification of Camellia sinensis
Proanthocyanidin-Aluminum Complexes Improve Aluminum Resistance and Detoxification of Camellia sinensis
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原花青素·铝络合物提高茶树的抗铝性和解毒能力
DOI：
10.1021/acs.jafc.0c01689
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发表时间：
2020-07-29
影响因子：
6.1
通讯作者：
Xia, Tao
中科院分区：
文献类型：
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作者：
Fu, Zhouping;Jiang, Xiaolan;Xia, Tao
酸性土壤中铝对作物产量有影响。茶树（Camellia sinensis）具有很强的耐铝性，其叶片中含有大量的单体儿茶素，尤其是表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG），根部含有大量的聚合原花青素（polymer proanthocyanidins，rPA）。这些多酚在茶树抗AI中的作用尚不清楚。在这项研究中，我们观察到，这些多酚可以形成复合物与铝在体外，络合能力是积极的影响高溶液pH值（pH 5.8），多酚类型（rPA和EGCG），和高铝浓度。在Al-27核磁共振（NMR）实验中，rPA-Al和EGCG-Al复合物信号在体外和体内均能检测到。结果表明，在温室茶树和茶园茶树的根和老叶中分别检测到rPA-Al和EGCG-Al复合物。铝在幼苗中主要积累在根和老叶中，并主要以结合态存在于质外体中。这些结果表明，与茶多酚在体内形成的复合物在茶树抗铝中起着至关重要的作用。
Aluminum (Al) influences crop yield in acidic soil. The tea plant (Camellia sinensis) has high Al tolerance with abundant monomeric catechins in its leaves, especially epigallocatechin gallate (EGCG), and polymeric proanthocyanidins in its roots (rPA). The role of these polyphenols in the AI resistance of tea plants is unclear. In this study, we observed that these polyphenols could form complexes with Al in vitro, and complexation capacity was positively influenced by high solution pH (pH 5.8), polyphenol type (rPA and EGCG), and high Al concentration. In the Al-27 nuclear magnetic resonance (NMR) experiment, rPA-Al and EGCG-Al complex signals could be detected both in vitro and in vivo. The rPA-Al and EGCG-Al complexes were detected in roots and old leaves, respectively, of both greenhouse seedlings and tea garden plants. Furthermore, in seedlings, Al accumulated in roots and old leaves and mostly existed in the apoplast in binding form. These results indicate that the formation of complexes with tea polyphenols in vivo plays a vital role in Al resistance in the tea plant.