Fructose-rich diet leads to reduced aerobic capacity and to liver injury in rats
Fructose-rich diet leads to reduced aerobic capacity and to liver injury in rats
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DOI：
10.1186/1476-511x-11-78
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发表时间：
2012-06-19
影响因子：
4.5
通讯作者：
Rostom de Mello, Maria Alice
中科院分区：
文献类型：
--
作者：
Botezelli, Jose Diego;Cambri, Lucieli Teresa;Rostom de Mello, Maria Alice
本研究的主要目的是研究富果糖饮食对Wistar大鼠有氧能力的改变和代谢变化的表现。我们根据饮食将28只大鼠分为两组：对照组(C)（均衡饮食）和富含果糖的饮食组(F)。各组饲喂60 d （120 ~ 180 d）。我们在第120和180天进行胰岛素、葡萄糖和最低乳酸测试。实验结束时，对16只动物实施安乐死，分析其有氧能力、血清天冬氨酸转氨酶（AST）与丙氨酸转氨酶（ALT）比值、血清和肝脏甘油三酯浓度、血清和肝脏硫代巴比妥酸活性物质（TBARS）浓度、血清和肝脏过氧化氢酶和超氧化物歧化酶（SOD）活性以及肝细胞血红素-伊红组织学（HE）。对其余12只动物进行肝脏脂肪生成率分析。喂食富含果糖的食物的动物表现出有氧能力、葡萄糖耐量和胰岛素敏感性的降低，肝脏中甘油三酯和TBARS的浓度增加。果糖饲喂动物肝脏过氧化氢酶和超氧化物歧化酶活性降低。血清AST/ALT比值高于C组，提示肝损害，组织学证实肝损害。综上所述，富含果糖的饮食造成了动物肝脏损伤和胰岛素敏感性降低，这可能导致有害的代谢影响。
The main purpose of this research was to investigate the alterations in the aerobic capacity and appearance of metabolic alterations in Wistar rats fed on fructose-rich diet. We separated twenty-eight rats into two groups according to diet: a control group (C) (balanced diet) and a fructose-rich diet group (F). The animals were fed these diets for 60 d (d 120 to 180). We performed insulin, glucose as well as a minimum lactate test, at d 120 and 180. At the end of the experiment, sixteen animals were euthanized, and the following main variables were analysed: aerobic capacity, the serum aspartate aminotransferase (AST) to alanine aminotransferase (ALT) ratio, serum and liver triglyceride concentrations, serum and liver thiobarbituric acid reactive substance (TBARS) concentrations, serum and liver catalase and superoxide dismutase (SOD) activity and haematoxylin-eosin histology (HE) in hepatocytes. The remaining twelve animals were submitted to an analysis of their hepatic lipogenic rate. The animals fed a fructose-rich diet exhibited a reduction in aerobic capacity, glucose tolerance and insulin sensitivity and increased concentrations of triglycerides and TBARS in the liver. Catalase and SOD activities were reduced in the livers of the fructose-fed animals. In addition, the serum AST/ALT ratio was higher than that of the C group, which indicates hepatic damage, and the damage was confirmed by histology. In conclusion, the fructose-rich diet caused significant liver damage and a reduction in insulin sensitivity in the animals, which could lead to deleterious metabolic effects.