Characterization of phytotoxin and secreted proteins identifies of Lasiodiplodia theobromae, causes of peach gummosis
Characterization of phytotoxin and secreted proteins identifies of Lasiodiplodia theobromae, causes of peach gummosis
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植物毒素和分泌蛋白的特征鉴定了可可毛二孢菌（Lasiodiplodia theobromae），这是桃树流胶病的原因。
DOI：
10.1016/j.funbio.2018.11.001
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发表时间：
2019-01-01
期刊：
影响因子：
2.5
通讯作者：
Li, Guohuai
中科院分区：
文献类型：
--
作者：
Li, Zhi;Zhang, He;Li, Guohuai
可可毛二孢是桃流胶病的病原真菌，是世界范围内桃生产的主要限制因子之一。本文报道了该菌毒性代谢产物的特征及分泌蛋白质组学研究。从其离体培养物中分离可可碱。L.理查德液体培养基培养滤液中的可可碱对桃当年生枝条的毒力较高，病斑直径大，胶重高。毒性测定结果表明，乙酸乙酯和二氯甲烷提取物分别诱导桃苗产生23.6和21.2 mg胶质，与其他有机溶剂相比差异显著。采用液相色谱-质谱联用技术从L.可可碱。序列分析表明，根据信号肽和定位，14个蛋白为胞外蛋白。从接种后第1天到第2天，所分析的真菌基因的表达谱显著上调，表明早期阶段是L.可可碱。本研究为桃流胶病病原菌胞外毒素及分泌蛋白的研究提供了新的思路。(C)2018年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
Lasiodiplodia theobromae, a phytopathogenic fungus that causes peach gummosis, is considered one of the major constraints to peach production worldwide. Here, we report the characteristics of toxic metabolites and the proteomits investigation of the secreted proteins of L. theobromae from its in vitro culture. The phytotoxins of L. theobromae from the culture filtrate of Richard's liquid medium showed high toxicity on peach current year shoots with large lesion diameter and high gum weight. The toxicity measurement showed that 23.6 and 21.2 mg gum were induced from peach shoots by solvent fractions of ethyl acetate and dichloromethane, respectively, with significant differences from other organic solvents. A total of 23 proteins were identified by liquid chromatography-mass spectrometry from the in vitro secretome of L. theobromae. Sequence analysis indicated that 14 proteins were extracellular proteins based on signal peptides and localization. The expression profiles of the analyzed fungal genes were significantly upregulated from 1 day postinoculation (dpi) to 2 dpi, indicating that the early stage is an important stage for the infection of L. theobromae. The present study has provided insights into the extracellular phytotoxins and secreted proteins that are possibly associated with pathogenicity of the peach gummosis. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved.