Electron microscopical investigation of surface alterations on Giardia lamblia trophozoites after exposure to a cytotoxic monoclonal antibody
Electron microscopical investigation of surface alterations on Giardia lamblia trophozoites after exposure to a cytotoxic monoclonal antibody
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DOI：
10.1007/s004360050096
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发表时间：
1996-03-01
影响因子：
2
通讯作者：
Muller, N
中科院分区：
文献类型：
--
作者：
Hemphill, A;Stager, S;Muller, N
本研究描述了蓝氏贾第鞭毛虫克隆GS/M-83-H7的滋养体暴露于单克隆抗体（MAb）G10/4后的透射电子显微镜调查。从以前的研究中，已知该抗体与寄生虫的主要表面抗原VSP（可变表面蛋白）发生免疫反应，并对克隆GS/M-83-H7的滋养体表现出补体非依赖性细胞毒性作用。我们的研究表明，MAb G10/4的细胞毒性与从寄生虫表面脱落的含VSP的膜囊泡和伴随的细胞膜的部分破坏有关。这些形态学改变取决于抗体的交联能力，因为相应单价F（ab）'的免疫反应性对细胞表面结构没有显著影响。这些发现表明，MAb G10/4的膜崩解活性可能是负责抗体的杀寄生虫功能。
The present study describes a transmission electron microscopical investigation of trophozoites from Giardia lamblia clone GS/M-83-H7 after exposure to monoclonal antibody (MAb) G10/4. From previous studies it is known that this antibody immunoreacts with the parasite's major surface antigen VSP (variable surface protein) and exhibits a complement-independent cytotoxic effect on trophozoites of clone GS/M-83-H7. Our investigations revealed that cytotoxicity of MAb G10/4 is associated with shedding of VSP-containing membrane vesicles from the parasite surface and a concomitant partial disruption of the cellular membrane. These morphological alterations depend on the cross-linking capacity of the antibody because the immunoreactivity of respective monovalent F(ab)' has no significant influence on the cell-surface structure. These findings indicate that the membrane-disintegrative activity of MAb G10/4 may be responsible for the parasitocidal function of the antibody.