Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases
Two-dimensional electrophoretic patterns of acute-phase human serum proteins in the course of bacterial and viral diseases
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DOI：
10.1002/elps.1150170333
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发表时间：
1996-03-01
期刊：
影响因子：
2.9
通讯作者：
Pallini, V
中科院分区：
文献类型：
--
作者：
Bini, L;Magi, B;Pallini, V
急性期血清蛋白质进行了分析，在3-10固定化pH梯度的双向电泳等电聚焦。大多数斑点通过参考SWISS-2DPAGE数据库中的血浆图谱来鉴定。用特异性抗血清免疫印迹和电泳参数预测值匹配确定血清淀粉样蛋白A蛋白斑点。铝-抗胰蛋白酶，富含亮氨酸的糖蛋白，结合珠蛋白，血清视黄醇结合蛋白和甲状腺素运载蛋白的浓度的变化进行了定量的银染凝胶的光密度法。比较了18例细菌性疾病和16例病毒性疾病的电泳图谱。血清淀粉样蛋白A斑点在细菌性疾病中的发生率为18/18，在病毒性疾病中的发生率为6/16。至于研究的其他反应物，在细菌性疾病中变化是同时的，在病毒性疾病中往往是交错的。
Acute-phase serum proteins were analyzed by two-dimensional electrophoresis with isoelectric focusing in 3-10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS-2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of al-antitrypsin, leucine-rich glycoprotein, haptoglobin, serum retinol-binding protein and transthyretin were quantitated by densitometry of silver-stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As for the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.