Biofilm formation of Candida albicans on implant overdenture materials and its removal
Biofilm formation of Candida albicans on implant overdenture materials and its removal
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DOI：
10.1016/j.jdent.2012.04.026
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发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Ichikawa, Tetsuo
中科院分区：
文献类型：
--
作者：
Li, Jing;Hirota, Katsuhiko;Ichikawa, Tetsuo
目的：本研究的目的是澄清各种种植覆盖义齿材料的表面特性和白色念珠菌粘附和生物膜形成的能力，并探讨唾液粘蛋白在生物膜形成中的作用。方法：7种常用的种植和修复材料进行了评估。所有材料的表面粗糙度平均值限制在0.07-0.10 μ m。测量接触角和唾液粘蛋白吸收。在90分钟的初始粘附和2天的生物膜形成后，C.采用菌落形成单位计数法测定白念珠菌的数量，扫描电镜观察其形态特征。结果：材料的表面接触角（疏水性指标）与C.白色念珠菌粘蛋白的吸收与生物膜的去除率呈负相关，表明粘蛋白在生物膜的形成及其刚性中起重要作用。结论：不同疏水性和组成的材料对唾液粘蛋白的吸附、初始粘附和生物膜形成的方式不同。疏水性材料促进增强的初始粘附，随后导致活性生物膜形成。粘蛋白对念珠菌在材料上的固定和生物膜的形成具有决定性的作用。临床意义：材料表面的亲水性和组成以及唾液蛋白，特别是粘蛋白，影响念珠菌生物膜的形成过程，影响生物膜的数量和硬度。本研究结果可为种植覆盖义齿材料的选择提供参考。(C)2012爱思唯尔有限公司保留所有权利。
Objectives: The purposes of this study were to clarify the surface characteristics of various implant overdenture materials and the capabilities of Candida albicans adherence and biofilm formation on these surfaces, and to investigate the role of salivary mucin in biofilm formation.Methods: Seven commonly used implant and restorative materials were assessed. The surface roughness averages of all materials were limited to 0.07-0.10 mu m. Contact angles and salivary mucin absorption were measured. After 90-min initial adhesion and 2-day biofilm formation, the amounts of C. albicans were determined by counting colony-forming units and the morphological characteristics were observed by scanning electron microscopy (SEM). The effects of saliva coating and the influences of material surface property on initial adhesion, biofilm formation and its removability were analysed by univariate two-way analysis of variance and multiple linear regression analysis.Results: Surface contact angle of materials, the index of hydrophobicity, was found to be correlated positively with initial adhesion and biofilm formation of C. albicans. A negative correlation between mucin absorption and removability of Candida biofilm indicates that mucin plays an important role in biofilm formation and its rigidity. SEM observation also revealed fewer Candida cells on saliva-coated Ti than on saliva-coated hydroxyapatite or acrylic resin.Conclusions: The materials with different hydrophobic property and compositions display diverse manners of salivary mucin absorption, initial adhesion and biofilm formation. The hydrophobic materials encourage enhanced initial adhesion, subsequently resulting in the active biofilm formation. Mucin has decisive effects on Candida immobilization and biofilm development on the materials.Clinical significance: Surface hydrophilic property and composition of materials and salivary proteins, especially mucin, affect the process of Candida biofilm formation and influence the amount and rigidity of formed biofilm. The present data may be applied as a reference for selecting materials in implant overdenture treatment from a microbiological point of view. (C) 2012 Elsevier Ltd. All rights reserved.