Antibacterial activity of the essential oil of Picea excelsa on Listeria, Staphylococcus aureus and coliform bacteria
Antibacterial activity of the essential oil of Picea excelsa on Listeria, Staphylococcus aureus and coliform bacteria
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DOI：
10.1006/fmic.2000.0397
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发表时间：
2001-06-01
影响因子：
5.3
通讯作者：
Mourey, A
中科院分区：
文献类型：
--
作者：
Canillac, N;Mourey, A
采用稀释法测定了云杉挥发油对1株伊氏李斯特菌、6株乳酸杆菌的抑菌活性。单核细胞增多症，三个金黄色葡萄球菌，三个大肠杆菌，一个产酸克雷伯氏菌，一个肺炎克雷伯氏菌肺炎亚种。肺炎杆菌和一种阴沟肠杆菌。对于处于稳定期的革兰氏阳性菌，0.07%的精油每ml抑制约10（5）个菌落形成单位，0.2-0.3%的剂量在37 ℃下对1 ml液体培养物中含有的10（5）-10（7）个细胞具有杀菌作用。浓度为10（5）CFU/ml的大肠菌群无论生理年龄如何都具有抗性，因为它们与8%的精油一起生长。简化的技术对于测定对李斯特菌的杀菌活性给出了良好的结果，仅指示对S.金黄色。(C)北京：科学出版社.
Antibacterial activity of essential oil of Picea excelsa was tested with the dilution method against one strain of Listeria ivanovii, six of L. monocytogenes, three of Staphylococcus aureus, three of Escherichia coli, one of Klebsiella oxytoca, one of K, pneumoniae subsp. pneumoniae and one of Enterobacter cloacae. For Gram-positive bacteria in stationary phase, 0.07% of essential oil inhibited about 10(5) colony forming unit per ml a dose of 0.2-0.3% was bactericidal for 10(5)-10(7) Cells contained in 1ml of liquid culture at 37 degrees C. The coliforms, at a concentration of 10(5) CFU per ml are resistant whatever the physiological age, since they grow with 8% of essential oil A simplified technique gives good results for the determination of bactericidal activity against Listeria, only indication against S. aureus. (C) 2001 Academic Press.