Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues
Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues
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DOI：
10.1152/physiolgenomics.2000.4.1.75
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发表时间：
2000-11-09
影响因子：
4.6
通讯作者：
Gross, KW
中科院分区：
文献类型：
--
作者：
Jones, CA;Hurley, MI;Gross, KW
将4kb肾素5′侧序列与人源化绿色荧光蛋白（GFP） cDNA融合，构建报告基因。产生了携带该构建体的转基因小鼠，并检测了GFP的表达。在成人中，在肾脏肾小球旁（JG）细胞和颌下腺颗粒状卷曲小管细胞中检测到表达。此外，卡托普利可诱导小鼠肾血管平滑肌细胞中GFP的表达。在胚胎发生过程中，在胚胎第13天的肾上腺和Wolffian导管中首次检测到GFP的表达。早在14岁时，在发育中的肾血管中也可见到表达，并且在出生时仍可检测到。成人肾GFP的表达仅限于JG细胞。胎儿肾上腺和性腺动脉也表达GFP。在胎盘中，在巨细胞滋养层细胞中观察到GFP，与人类和小鼠绒毛膜细胞中肾素表达的报道一致。我们得出结论，4 kb的肾素5'侧翼足以指导多种已知的肾素表达模式。此外，本文描述的肾素- gfp结构将为肾素表达提供有用的重要报告因子。
A reporter construct was assembled with 4-kb of renin 5'-flanking sequence fused to humanized green fluorescent protein (GFP) cDNA. Transgenic mice carrying this construct were produced and assayed for GFP expression. In the adult, expression was detected in juxtaglomerular (JG) cells of the kidney and granular convoluted tubular cells of the submandibular gland. Furthermore, treatment of mice with captopril induced GFP expression in renal vascular smooth muscle cells. During embryogenesis, GFP expression was first detected at embryonic day E13 in the adrenal gland and Wolffian duct. Expression was also seen in the developing renal vasculature as early as E14 and remained detectable through birth. Renal GFP expression became restricted to JG cells in adults. Fetal adrenal and gonadal arteries also expressed GFP. In the placenta, GFP was observed in giant cell trophoblasts, consistent with reports of renin expression in chorionic cells of both humans and mice. We conclude that 4 kb of renin 5' flank is sufficient to direct multiple known renin expression patterns. Furthermore, the renin-GFP construct characterized here will provide a useful vital reporter for renin expression.