STUDY OF PENETRATION OF MAMMALIAN CELLS BY DEOXYRIBONUCLEIC ACIDS
STUDY OF PENETRATION OF MAMMALIAN CELLS BY DEOXYRIBONUCLEIC ACIDS
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DOI：
10.1083/jcb.9.1.81
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发表时间：
1961-01-01
期刊：
影响因子：
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通讯作者：
BENDICH, A
中科院分区：
文献类型：
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作者：
BORENFREUND, E;BENDICH, A
将来自肺炎球菌和人类白细胞的氚标记的脱氧核糖核酸（DNA）加入到37 ℃下生长的HeLa细胞培养物中。放射自显影显示氚在核区域有广泛的定位。用核糖核酸酶或稀高氯酸处理不能除去标记，但用脱氧核糖核酸酶可以从细胞中定量除去。从暴露的HeLa中分离的核酸的化学和放射性测定。细胞显示在所有4个DNA碱基中存在氚。约12 [mu]g。从暴露于240 [mu]g 24小时的6 X 106个HeLa细胞中回收了30 μ g的氚化DNA。人类的DNA。由此可以得出结论，细胞吸收的DNA或其降解产物的量相当于正常HeLa细胞补体的至少10%。
Tritium-labeled deoxyribonucleic acid (DNA) from pneumococci and from human leukocytes was added to growing cultures of HeLa cells at 37[degree]C. Autoradio-graphy revealed an extensive localization of tritium in the nuclear regions. The label could not be removed by treatment with ribo-nuclease or dilute perchloric acid, but quantitative removal from the cells could be effected with deoxyribonuclease. Chemical and radioactivity determinations on nuclei acids isolated from the exposed HeLa. cells revealed the presence of tritium in all 4 DNA bases. About 12 [mu]g. of tritiated DNA was recovered from 6 X 106 HeLa cells which had been exposed for 24 hours to 240 [mu]g. of the human DNA. From this, it is concluded that the amount of DNA, or its degradation products, taken up by the cells was equivalent to at least 10 per cent of the normal HeLa cell complement.