Use of gallocyanin as a myelin stain for brain and spinal cord.
Use of gallocyanin as a myelin stain for brain and spinal cord.
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使用没食子蓝蛋白作为大脑和脊髓的髓磷脂染色剂。
DOI：
10.3109/10520297109067838
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
J. Sepinwall
中科院分区：
文献类型：
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作者：
V. Augulis;J. Sepinwall
将组织固定在10%福尔马林、福尔马林盐水、CaCO3或磷酸盐缓冲液中和福尔马林、Baker’s formol钙、Cajal’s formol溴化铵、福尔马林-95%乙醇1:9、福尔马林-甲醇1:9、Lillie’s甲醇-氯仿或Salthouse’s formol十六烷基三甲基溴化铵中脱水并包埋在石蜡中。切片用蛋白或明胶胶粘剂贴在载玻片上，在室温22-25℃下进行处理，在1%铁明矾中浸泡30-60分钟，然后在4次蒸馏水中洗涤10分钟。髓磷脂在半胱甘肽自分化液中染色1-2.5小时；厚的部分需要更长的时间。染色液（pH约7.4）由Na2CO3, 90 mg组成；蒸馏水，100毫升；半胱氨酸，250毫克；乙醇，5ml。最后加入乙醇，其他成分煮沸后冷却至室温。染色和彻底清洗后，尼氏颗粒染色5-10英里。
Tissue fixed in 10% formalin, formol saline, CaCO3 or phosphate buffer neutralized formalin, Baker's formol calcium, Cajal's formol ammonium bromide, formalin-95% ethanol 1:9, formalin-methanol 1:9, Lillie's methanol-chloroform or Salthouse's formol cetyltrimethylammonium bromide was dehydrated and embedded in paraffin. Sections were attached to slides with either albumen or gelatine adhesive and processed throughout at room temperature of 22–25 C. Mordanting 30–60 min in 1% iron alum was followed by a 10 min wash in 4 changes of distilled water. Myelin was stained in a gallocyanin self-differentiating solution for 1–2.5 hr; thick sections requiring the longer time. The staining solution (pH approximately 7.4) consisted of Na2CO3, 90 mg; distilled water, 100 ml; gallocyanin, 250 mg; and ethanol, 5 ml. The ethanol was added to this mixture last, and after the other ingredients had been boiled and then cooled to room temperature. After a staining and thorough washing, Nissl granules were stained for 5–10 mi...