Biological function of metallothionein. V. Its induction in rats by various stresses.
Biological function of metallothionein. V. Its induction in rats by various stresses.
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金属硫蛋白的生物学功能。
DOI：
10.1152/ajpendo.1978.234.3.e282
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
P. Weswig
中科院分区：
文献类型：
--
作者：
S. Oh;J. Deagen;P. Whanger;P. Weswig
研究了以下应激条件下大鼠肝脏和肾脏中金属硫蛋白（一种锌结合蛋白）的诱导：冷环境、热环境、热烧伤、剧烈运动和CCl 4中毒。以~（14）C胱氨酸和~（65）Zn作为金属硫蛋白（MT）合成的标志物，经大鼠胃肠道外注射，用SephadexG-75柱分离肝、肾上清液中的MT。在测试的应力中，在寒冷（315%）、剧烈运动（302%）和CCl 4中毒（543%）下观察到肝脏MT合成的最大刺激，这通过[14 C]胱氨酸和65 Zn掺入MT组分的量增加来证明。与肝脏相反，在肾脏中的MT合成中仅观察到小的响应。有一个普遍的下降，血浆锌水平在大鼠的应力下。然而，在整个肝脏和肾脏中没有观察到很大的差异。本研究表明，各种无关的生理应激，这是伴随着锌分布的变化，增加MT在大鼠肝脏的合成。
Induction of metallothionein (a Zn-binding protein) was investigated in liver and kidneys of rats under the following stresses: cold environment, hot environment, heat burn, strenuous exercise, and CCl4 intoxication. As markers of metallothionein (MT) synthesis, [14C]cystine and 65Zn were injected parenterally into rats, and MT in liver and kidney supernatants was separated on Sephadex G-75 columns. Among stresses tested, the greatest stimulations of MT synthesis in liver were observed under cold (315%), strenuous exercise (302%), and CCl4 intoxication (543%), as evidenced by increased amount of [14C]cystine and 65Zn incorporation into the MT fraction. In contrast to liver, only small responses were observed in MT synthesis in kidneys. There was a general decline in plasma Zn levels in rats under the stresses employed. However, no great differences were observed in Zn levels in whole liver and kidneys. The present study suggests that various unrelated physiological stresses, which are accompanied with a change in Zn distribution, increase the synthesis of MT in liver of rats.