Dissipation of the proton electrochemical potential in intact and lysed chloroplasts : I. The electrical potential.
Dissipation of the proton electrochemical potential in intact and lysed chloroplasts : I. The electrical potential.
复制标题
完整和裂解叶绿体中质子电化学势的耗散：I. 电势。
作者：
J. Nishio;J. Whitmarsh
有效的离子载体：叶绿素的比例，确定了各种离子载体，减少跨类囊体膜的完整和hypo-atmosphically裂解的叶绿体分离菠菜（菠菜）的电位。短杆菌肽D、缬氨霉素、羰基氰间氯苯腙和二环己基-18-冠-6在瓦解电势中的功效通过饱和单次翻转闪光诱导的518纳米处的吸光度变化的衰减半衰期以电化学计量法测定。结果表明，在崩溃的完整的和裂解的叶绿体中的电位的离子载体的有效性取决于在测定介质中的离子载体可接近的膜的量。只有短杆菌肽在完整的和裂解的叶绿体之间表现出显著的功效差异。在完整的叶绿体中，短杆菌肽与叶绿素的比例比裂解的叶绿体高50倍以上。在牛血清白蛋白存在下，羰基氰间氯苯腙的效力显著降低。在牛血清白蛋白的存在下，测试的其他离子载体保持其效力。缬氨霉素是最有效的离子载体崩溃完整的叶绿体中的电位测试，而短杆菌肽是最有效的离子载体裂解的叶绿体。折叠的电势所需的离子载体：叶绿素的比例的意义进行了讨论，关于生物能量的研究，特别是那些检查的跨膜电化学电位到叶绿体的蛋白质运输的贡献。
Effective ionophore:chlorophyll ratios were determined for various ionophores that decrease the electrical potential across thylakoid membranes in intact and hypo-osmotically lysed chloroplasts isolated from spinach (Spinacia oleracea). The efficacy of gramicidin D, valinomycin, carbonylcyanide m-chlorophenylhydrazone, and dicyclohexano-18-crown-6 in collapsing the electrical potential was determined spectrophotometrically by the decay half-time of the absorbance change at 518 nanometers induced by a saturating, single turnover flash. The results show that the effectiveness of the ionophores in collapsing the electrical potential in intact and lysed chloroplasts depends on the amount of ionophore-accessible membrane in the assay medium. Only gramicidin exhibited a significant difference in efficacy between intact and lysed chloroplasts. The ratio of gramicidin to chlorophyll required to collapse the electrical potential was more than 50 times higher in intact chloroplasts than in lysed chloroplasts. The efficacy of carbonylcyanide m-chlorophenylhydrazone was significantly reduced in the presence of bovine serum albumin. The other ionophores tested maintained their potency in the presence of bovine serum albumin. Valinomycin was the most effective ionophore tested for collapsing the electrical potential in intact chloroplasts, whereas gramicidin was the most potent ionophore in lysed chloroplasts. The significance of the ionophore:chlorophyll ratios required to collapse the electrical potential is discussed with regard to bioenergetic studies, especially those that examine the contribution of the transmembrane electrochemical potential to protein transport into chloroplasts.
将不完全折叠的前体蛋白导入分离的线粒体需要通电的内膜，但不需要添加 ATP。
DOI：
10.1002/j.1460-2075.1987.tb02524.x
发表时间：
1987
期刊：
The EMBO journal
影响因子：
--
作者：
Verner,K;Schatz,G
通讯作者：
Schatz,G
棕榈酸和短杆菌肽 D 引起的光磷酸化异常解偶联。
影响因子：
2.9
作者：
Pick,U;Weiss,M;Rottenberg,H
通讯作者：
Rottenberg,H