Rapid ionic events and the initiation of growth in serum-stimulated neuroblastoma cells
Rapid ionic events and the initiation of growth in serum-stimulated neuroblastoma cells
复制标题
血清刺激的神经母细胞瘤细胞中的快速离子事件和生长起始
DOI：
10.1016/0092-8674(81)90443-8
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发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
S. W. Laat
中科院分区：
文献类型：
--
作者：
W. Moolenaar;C. Mummery;P. Saag;S. W. Laat
在小鼠N1 E-115神经母细胞瘤细胞中研究了血清刺激对电和离子膜特性的快速影响及其与DNA合成和细胞分裂起始的关系。向去血清的N1 E115细胞中加入10%胎牛血清导致在大约10小时的滞后后开始DNA合成。加入血清后最早的事件包括：瞬时膜电位和电阻变化，在几秒钟内可检测到并持续5- 10分钟;持续增加的初始速率22 Na+流入，其中大部分不是电扩散起源，这是由弱酸性阴离子增强;以及Na+，K+泵速率的外部Na+依赖性增加。在不存在血清的情况下，可以通过用莫能菌素或神经毒素增加净Na+内流来模拟Na+、K+泵的刺激。生长耗尽血清不能诱导任何电和离子事件。利尿剂阿米洛利（0.4 mM）抑制血清诱导的Na+内流，Na+，K+泵刺激和DNA合成，但不影响电反应或基础内流率。结果表明，血清生长因子的行为，至少部分，通过刺激电中性，amiloridesensitive Na+/H+交换机制。增强的Na+内流然后导致观察到的Na+、K+泵的刺激，而同时质子的流出可升高细胞内pH。
Rapid effects of serum stimulation on electrical and ionic membrane properties and their relationship to the initiation of DNA synthesis and cell division have been investigated in mouse NlE-115 neuroblastoma cells. Addition of 10% fetal calf serum to serum-deprived Nl El 15 cells results in the initiation of DNA synthesis after a lag of approximately 10 hr. The earliest events following serum addition include: transient membrane potential and resistance changes, detectable within seconds and lasting 5-l 0 min; a persistent increase in the initial rate of 22Na+ influx, the major part of which is not of electrodiffusional origin, and which is potentiated by weak acid anions; and an external Na+-dependent increase in the rate of the Na+, K+ pump. In the absence of serum the stimulation of the Na+, K+ pump can be mimicked by increasing net Na+ influx with monensin or neurotoxins. Growth-depleted serum fails to induce any of the electrical and ionic events. The diuretic amiloride (0.4 mM) inhibits serum-induced Na+ influx, Na+, K+ pump stimulation and DNA synthesis, but does not affect the electrical response or the basal influx rates. The results suggest that serum growth factors act, at least in part, by stimulating an electroneutral, amiloridesensitive Na+/H+ exchange mechanism. The enhanced Na+ influx then results in the observed stimulation of the Na+, K+ pump, while the simultaneous efflux of protons may raise the intracellular PH.