Hydrogen sulfide-induced chilling resistance in peach fruit is performed via sustaining the homeostasis of ROS and RNS.
Hydrogen sulfide-induced chilling resistance in peach fruit is performed via sustaining the homeostasis of ROS and RNS.
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DOI：
10.1016/j.foodchem.2022.133940
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发表时间：
2022-08
期刊：
影响因子：
8.8
通讯作者：
Yaqing Zhao;Liangyi Zhao;Shunqing Hu;Y. Hou;Jingdao Wang;Yonghua Zheng;P. Jin
中科院分区：
文献类型：
--
作者：
Yaqing Zhao;Liangyi Zhao;Shunqing Hu;Y. Hou;Jingdao Wang;Yonghua Zheng;P. Jin
研究了硫化氢（H2S）对冷藏桃果实内源H2S、活性氧（ROS）和活性氮（RNS）的影响。20 μL L− 1H 2S（NaHS作为供体）有效地延缓了冷害（CI）引起的品质劣变，引发内源H2S的产生，同时增强了抗氧化系统和ROS（NADPH氧化酶）的产生。H2S促进一氧化氮（NO）与S-亚硝基谷胱甘肽还原酶（GSNOR）介导的GSNO还原，同时抑制过氧亚硝酸根阴离子含量。烟酰胺腺嘌呤二核苷酸磷酸（nicotinamide adenine dinucleotide phosphate，NADPH）是ROS和RNS的关键辅酶，在贮藏后期，H2S通过三羧酸循环升高NADPH水平，降低NADP-ICDH活性和基因表达。桃NADP-ICDH（UniProtKB M5 WXP 5）的结构分析推断Cys 79和Tyr 396分别是S-亚硝基化和硝化的最可能的靶标。说明H_2S通过抑制活性氧和RNS的失调来改善桃果实的CI。
Hydrogen sulfide (H2S) application impacts on endogenous H2S, reactive oxygen species (ROS), and reactive nitrogen species (RNS) in cold-stored peach fruit were investigated. 20 μL L−1H2S (NaHS as the donor) efficiently retarded the quality deterioration arising from chilling injury (CI), triggering endogenous H2S production, while enhancing antioxidant systems and ROS generation (NADPH oxidative enzyme). H2S promoted nitric oxide (NO) correlated with the S-nitrosoglutathione reductase (GSNOR)-mediated GSNO reduction, while suppressing the peroxynitrite anion content. As the pivotal coenzyme of ROS and RNS, nicotinamide adenine dinucleotide phosphate (NADPH) levels were elevated by H2S during late-stage storage via the tricarboxylic acid cycle, where reduced NADP-isocitrate dehydrogenase (NADP-ICDH) activity and gene expression. Structural analysis of peach NADP-ICDH (UniProtKB M5WXP5) deduced that Cys79 and Tyr396 are the likeliest targets for S-nitrosylation and nitration, respectively. These results indicate that H2S counteracts the disorders of ROS and RNS to ameliorate CI of peach fruit.