NEUROGENESIS AND ASTROGENESIS CONTRIBUTION TO RECOVERY OF VESTIBULAR FUNCTIONS IN THE ADULT CAT FOLLOWING UNILATERAL VESTIBULAR NEURECTOMY: CELLULAR AND BEHAVIORAL EVIDENCE
NEUROGENESIS AND ASTROGENESIS CONTRIBUTION TO RECOVERY OF VESTIBULAR FUNCTIONS IN THE ADULT CAT FOLLOWING UNILATERAL VESTIBULAR NEURECTOMY: CELLULAR AND BEHAVIORAL EVIDENCE
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DOI：
10.1016/j.neuroscience.2009.09.048
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发表时间：
2009-12-29
期刊：
影响因子：
3.3
通讯作者：
Tighilet, B.
中科院分区：
文献类型：
--
作者：
Dutheil, S.;Brezun, J. M.;Tighilet, B.
在生理条件下，神经发生发生在成年哺乳动物大脑的限制区域，产生整合成功能网络的神经元。在病理或损伤后条件下，也可能发生神经发生和星形发生，如成年猫立即单侧前庭神经切除术（UVN）后去传入前庭核所示。为了确定UVN后的反应性细胞增殖以及神经发生和星形发生是否在前庭功能恢复中起作用，我们检查了抗有丝分裂药物的作用：胞嘧啶-β-D阿拉伯呋喃糖苷（AraC），在UVN后注入第四脑室。可塑性机制在免疫组化水平证明溴脱氧尿苷，GAD 67和胶质细胞酸性蛋白（GFAP）染色。立即或延迟AraC输注的行为恢复过程的后果进行了评估与oculabetry和姿势运动测试。我们报道了UVN后立即注入AraC可阻断神经切断猫前庭神经核的细胞增殖，并减少GFAP免疫反应阳性细胞和GABA能神经元的数量。在行为水平上，UVN和立即AraC输注后，姿势运动功能恢复的时间过程显着延迟，水平自发性眼球震颤没有观察到改变。相反，UVN后3周开始输注AraC对行为恢复的时间过程没有影响，也没有影响反应性细胞增殖及其分化。我们的结论是，UVN后的前3周代表了一个可能的关键时期，在此期间发生了重要的神经可塑性机制，以促进前庭功能恢复：反应性神经发生和星形发生可能对成年猫的前庭补偿有很大贡献。（C）2009 IBRO。由爱思唯尔有限公司出版。保留所有权利。
In physiological conditions, neurogenesis occurs in restricted regions of the adult mammalian brain, giving rise to integrated neurons into functional networks. In pathological or postlesional conditions neurogenesis and astrogenesis can also occur, as demonstrated in the deafferented vestibular nuclei after immediate unilateral vestibular neurectomy (UVN) in the adult cat. To determine whether the reactive cell proliferation and beyond neurogenesis and astrogenesis following UVN plays a functional role in the vestibular functions recovery, we examined the effects of an antimitotic drug: the cytosine-beta-D arabinofuranoside (AraC), infused in the fourth ventricle after UVN. Plasticity mechanisms were evidenced at the immunohistochemical level with bromodeoxyuridine, GAD67 and glial fibrillary acidic protein (GFAP) stainings. Consequences of immediate or delayed AraC infusion on the behavioral recovery processes were evaluated with oculomotor and posturo-locomotor tests. We reported that after UVN, immediate AraC infusion blocked the cell proliferation and decreased the number of GFAP-immunoreactive cells and GABAergic neurons observed in the vestibular nuclei of neurectomized cats. At the behavioral level, after UVN and immediate AraC infusion the time course of posturo-locomotor function recovery was drastically delayed, and no alteration of the horizontal spontaneous nystagmus was observed. In contrast, an infusion of AraC beginning 3 weeks after UVN had no influence neither on the time course of the behavioral recovery, nor on the reactive cell proliferation and its differentiation. We conclude that the first 3 weeks after UVN represent a possible critical period in which important neuroplasticity mechanisms take place for promoting vestibular function recovery: reactive neurogenesis and astrogenesis might contribute highly to vestibular compensation in the adult cat. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.