The victorin-induced mitochondrial permeability transition precedes cell shrinkage and biochemical markers of cell death, and shrinkage occurs without loss of membrane integrity
The victorin-induced mitochondrial permeability transition precedes cell shrinkage and biochemical markers of cell death, and shrinkage occurs without loss of membrane integrity
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DOI：
10.1111/j.1365-313x.2004.02040.x
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发表时间：
2004-04-01
期刊：
影响因子：
7.2
通讯作者：
Wolpert, TJ
中科院分区：
文献类型：
--
作者：
Curtis, MJ;Wolpert, TJ
在这项研究中，我们确定了与宿主选择性毒素victorin诱导的细胞死亡相关的事件的时间。我们发现，维克托林诱导的线粒体跨膜电位（Δ Psi（m）），指示线粒体通透性转换（MPT），在每个细胞的基础上，崩溃，并没有同时发生在整个线粒体人口。Deltapsi（m）的损失在一个主要的人口线粒体细胞收缩前20-35分钟。Rubisco裂解，DNA梯状，和Victorin结合到P蛋白发生伴随着细胞收缩。在细胞收缩期间和之后，液泡膜破裂没有发生，膜，包括质膜和液泡膜，保持完整性。乙烯信号转导涉及维克托林诱导的线粒体运动性丧失和Deltapsi（m）崩溃的上游。结果表明，Victorin诱导的Deltapsi（m）的崩溃是MPT的结果，Victorin诱导的MPT的时间准备影响细胞死亡反应。细胞收缩期间质膜和液泡膜完整性的保留支持了Victorin诱导凋亡样细胞死亡反应的解释。
In this study, we determined the timing of events associated with cell death induced by the host-selective toxin, victorin. We show that the victorin-induced collapse in mitochondrial transmembrane potential (Deltapsi(m)), indicative of a mitochondrial permeability transition (MPT), on a per cell basis, did not occur simultaneously in the entire mitochondrial population. The loss of Deltapsi(m) in a predominant population of mitochondria preceded cell shrinkage by 20-35 min. Rubisco cleavage, DNA laddering, and victorin binding to the P protein occurred concomitantly with cell shrinkage. During and following cell shrinkage, tonoplast rupture did not occur, and membranes, including the plasma membrane and tonoplast, retained integrity. Ethylene signaling was implicated upstream of a victorin-induced loss in mitochondrial motility and the collapse in Deltapsi(m). Results suggest that the victorin-induced collapse in Deltapsi(m) is a consequence of an MPT and that the timing of the victorin-induced MPT is poised to influence the cell death response. The retention of plasma membrane and tonoplast integrity during cell shrinkage supports the interpretation that victorin induces an apoptotic-like cell death response.