Acetylene as an Inhibitor of Methanogenic Bacteria
Acetylene as an Inhibitor of Methanogenic Bacteria
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DOI：
10.1099/00221287-128-10-2453
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发表时间：
1982-10
期刊：
影响因子：
1.5
通讯作者：
G. Sprott;K. Jarrell;K. M. Shaw;R. Knowles
中科院分区：
文献类型：
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作者：
G. Sprott;K. Jarrell;K. M. Shaw;R. Knowles
总结：六个纯培养的产甲烷菌的生长被抑制低浓度的溶解乙炔（C2 H2）;其他古细菌（三个盐杆菌属物种）和几个真细菌没有类似的影响。完全抑制亨氏甲烷菌生长所需的最低溶解C_2H_2浓度约为8 μ m; 20 μ m的溶解乙烯对生长几乎没有影响。溶解乙炔（33 μ m）不改变培养基的Eh，也不导致M活力的丧失。暴露16 h后的Hungatei。在M. hungatei，氢化酶，NADP还原酶，甲基-辅酶M还原酶和ATP水解酶的活性不受C_2H_2浓度比生长抑制所需的浓度高几倍。所有的甲烷菌的细胞内ATP含量急剧下降，暴露于C2 H2。此外，M. hungatei和布氏甲烷杆菌在暴露于C2 H2时失去了维持跨膜pH梯度的能力。我们认为，暴露于C2 H2导致产甲烷菌功能下降，需要H+通量，包括ATP合成，Ni 2+吸收和甲烷生成。
SUMMARY: Growth of six pure cultures of methanogens was inhibited by low concentrations of dissolved acetylene (C2H2); other archaebacteria (three Halobacterium species) and several eubacteria were not similarly affected. The minimum concentration of dissolved C2H2 required to inhibit growth of Methanospirillum hungatei completely was about 8 mu;m; dissolved ethylene at 20 mu;m had little effect on growth. Dissolved acetylene (33 mu;m) did not alter the E h of the medium, or result in a loss in viability of M. hungatei after 16 h exposures. In anaerobic cell extracts of M. hungatei, activities of hydrogenase, NADP reductase, methyl-coenzyme M reductase and ATP hydrolase were not inhibited by C2H2 concentrations several times higher than those required for growth inhibition. The intracellular ATP content of all of the methanogens dropped dramatically on exposure to C2H2. Moreover, cells of M. hungatei and Methanobacterium bryantii on exposure to C2H2 lost their ability to maintain a transmembrane pH gradient. We suggest that exposure to C2H2 results in a decline in methanogen functions which require a H+-flux, including ATP synthesis, Ni2+ uptake and methanogenesis.