Purification of the sperm-binding factor from the egg of the sea urchin, Hemicentrotus pulcherrimus.
Purification of the sperm-binding factor from the egg of the sea urchin, Hemicentrotus pulcherrimus.
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从海胆 Hemicentrotus pulcherrimus 卵中纯化精子结合因子。
DOI：
10.1016/0006-291x(77)90753-7
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发表时间：
1977
影响因子：
3.1
通讯作者：
K. Aketa
中科院分区：
文献类型：
--
作者：
H. Tsuzuki;M. Yoshida;K. Onitake;K. Aketa
从海胆（Hemicentrotus pulcherrimus）的未受精卵中成功纯化了似乎负责受精时精子结合的物质。它完全取消了同源精子的精子能力，而不降低它们的活力。这种物质的抗血清只能使同源卵不能结合精子。纯化方法有尿素萃取法、醋酸钙分级法、硫酸铵盐析法、凝胶过滤法和离子交换层析法。该物质在醋酸纤维素试纸条上显示为单一条带，用酰胺黑染色，并且不能被6 M盐酸胍分裂。
The substance which seems to be responsible for the sperm-binding at fertilization was successfully purified from unfertilized eggs of the sea urchin, Hemicentrotus pulcherrimus. It completely cancelled the fertilizing capacity only of homologous sperm without reducing their motility. The antiserum against this substance made only homologous eggs incapable of binding sperm. The methods employed for purification were (1) extraction by urea,(2) fractionation by calcium acetate,(3) salting-out by ammonium sulfate,(4) gel filtration and (5) ion-exchange chromatography. This substance was electrophoresed on cellulose-acetate strip as a single band which was stained with Amido Black, and could not be split by 6 M guanidine hydrochloride.