Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation
Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation
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DOI：
10.1369/jhc.5a6650.2005
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发表时间：
2006-05-01
影响因子：
3.2
通讯作者：
Ameye, LG
中科院分区：
文献类型：
--
作者：
Goldberg, M;Septier, D;Ameye, LG
为了确定纤维调节蛋白（Fmod）（一种富含亮氨酸的硫酸角质蛋白多糖）在牙齿形成中的功能，我们通过免疫组织化学研究了 Fmod 在牙齿组织中的分布，并使用光学和透射电子显微镜表征了 1 日龄 Fmod 缺陷小鼠的牙齿表型。免疫组织化学方法还比较了Fmod基因缺陷小鼠和野生型小鼠之间牙本质唾液蛋白（DSP）、牙本质基质蛋白-1（DMP 1）、骨唾液蛋白（BSP）和骨桥蛋白（OPN）的相对蛋白表达。在正常小鼠和大鼠中，Fmod免疫染色主要在成牙本质细胞远端细胞体和中间层中检测到，在成牙本质细胞突起和前牙本质中较弱。 Fmod 的缺乏会损害牙本质矿化，增加整个牙本质前胶原纤维的直径，并延迟牙釉质的形成。免疫组织化学为 Fmod 缺陷小鼠的补偿机制提供了证据。臼齿中 DSP 和 OPN 的染色减少，而 DMP 1 和 BSP 的染色增强。在门牙中，牙髓和成牙本质细胞中的 DSP、DMP 1 和 BSP 标记强烈增加，而 OPN 染色减少。在分泌性成釉细胞中还发现 DMP 1 和 BSP 呈阳性染色。这些研究共同表明，Fmod 限制牙本质前胶原纤维的形成，同时促进牙本质矿化和牙釉质形成的早期阶段。
To determine the functions of fibromodulin (Fmod), a small leucine-rich keratan sulfate proteoglycan in tooth formation, we investigated the distribution of Fmod in dental tissues by immunohistochemistry and characterized the dental phenotype of 1-day-old Fmod-deficient mice using light and transmission electron microscopy. immunohistochemistry was also used to compare the relative protein expression of dentin sialoprotein (DSP), dentin matrix protein-1 (DMP 1), bone sialoprotein (BSP), and osteopontin (OPN) between Fmod-cleficient mice and wild-type mice. In normal mice and rats, Fmod immunostaining was mostly detected in the distal cell bodies of odontoblasts and in the stratum intermedium and was weaker in odontoblast processes and predentin. The absence of Fmod impaired dentin mineralization, increased the diameter of the collagen fibrils throughout the whole predentin, and delayed enamel formation. Immunohistochemistry provides evidence for compensatory mechanisms in Fmod-deficient mice. Staining for DSP and OPN was decreased in molars, whereas DMP 1 and BSP were enhanced. in the incisors, labeling for DSP, DMP 1, and BSP was strongly increased in the pulp and odontoblasts, whereas OPN staining was decreased. Positive staining was also seen for DMP 1 and BSP in secretory ameloblasts. Together these studies indicate that Fmod restricts collagen fibrillogenesis in predentin while promoting dentin mineralization and the early stages of enamel formation.