THE INTERACTION OF LACTOBACILLUS-ACIDOPHILUS AND TRICHOMONAS-VAGINALIS IN-VITRO
THE INTERACTION OF LACTOBACILLUS-ACIDOPHILUS AND TRICHOMONAS-VAGINALIS IN-VITRO
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DOI：
10.2307/3283344
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发表时间：
1994-02-01
影响因子：
1.3
通讯作者：
GARBER, GE
中科院分区：
文献类型：
--
作者：
MCGRORY, T;MEYSICK, K;GARBER, GE
最近对小鼠阴道毛滴虫感染模型的研究表明，用嗜酸乳杆菌预感染的雌激素化BALB/c小鼠表现出更长的T。与未用L.嗜酸乳杆菌研究T. vagelis和L.此外，我们在两个物种之间进行了体外竞争性生长测定。虽然增加了。嗜酸乳杆菌对T.阴道培养物减慢了原虫的生长，添加的细菌不增加滴虫的死亡。然而，T.迷走神经对L.当与匹配的对照相比时，在组合培养物中嗜酸乳杆菌生长。使用10（5）/ml的初始接种物，在40小时，对照L.嗜酸乳杆菌浓度已增长到1.3 × 10（7）/ml。然而，在与T.瓦格利湖嗜酸乳杆菌浓度在40小时时下降到7.8 × 10（5）/ml，10：1（T. 10（4））和1：1（T. 10（5））试验比值（P < 0.01）。这表明T.迷走神经可引起L.嗜酸乳杆菌下降，并可能解释了为什么L。滴虫病中阴道内嗜酸福尔斯落。
Recent work with a mouse model of Trichomonas vaginalis infection indicated that estrogenized BALB/c mice that were preinfected with Lactobacillus acidophilus showed a greater duration of T. vaginalis infection as compared to a control group of mice that were not treated with L. acidophilus. To examine the interaction between T. vaginalis and L. acidophilus further we performed in vitro competitive growth assays between the 2 species. Although the addition oft. acidophilus to the T. vaginalis cultures slowed the growth of the protozoa, the added bacteria did not increase trichomonad death. However, T. vaginalis had a deleterious effect on L. acidophilus growth in combined cultures when compared to matched controls. Using an initial inoculum of 10(5)/ml, at 40 hr the control L. acidophilus concentrations had grown to 1.3 x 10(7)/ml. However, in combined culture with T. vaginalis, L. acidophilus concentrations at 40 hr had fallen to 7.8 x 10(5)/ml and 6.1 x 10(4)/ml for the 10:1 (T. vaginalis at 10(4)) and 1:1 (T. vaginalis at 10(5)) test ratios, respectively (P < 0.01). This demonstrates that T. vaginalis can cause the concentration of L. acidophilus to fall in vitro and may explain why the concentration of L. acidophilus in the vagina falls in trichomoniasis.