Adenosine kinase gene expression in human colorectal cancer
Adenosine kinase gene expression in human colorectal cancer
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DOI：
10.1080/15257770802145629
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发表时间：
2008-01-01
影响因子：
1.3
通讯作者：
Vannoni, D.
中科院分区：
文献类型：
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作者：
Giglioni, S.;Leoncini, R.;Vannoni, D.
应用实时荧光定量逆转录聚合酶链反应（qRT-PCR）技术检测10例结直肠癌患者肠活检标本中腺苷代谢关键酶腺苷激酶的基因表达。定量mRNA表达水平相对于参考基因-肌动蛋白标准化。结果表明，腺苷激酶基因表达显着高于癌症比正常出现的组织，符合我们以前的测量腺苷激酶活性在结直肠肿瘤样本。
Real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate gene expression of adenosine kinase, a key enzyme in adenosine metabolism, in human intestinal biopsy specimens of 10 colorectal cancer patients. Quantitative mRNA expression levels were normalized against the reference gene -actin. The results showed that adenosine kinase gene expression was significantly higher in cancer than in normal-appearing tissue, in line with our previous measurements of adenosine kinase enzyme activities in colorectal tumor samples.