Molecular response of gall induction by aphid Schlechtendalia chinensis (Bell) attack on Rhus chinensis Mill
Molecular response of gall induction by aphid Schlechtendalia chinensis (Bell) attack on Rhus chinensis Mill
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DOI：
10.1080/17429145.2017.1392627
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发表时间：
2017-10-30
影响因子：
3.2
通讯作者：
Chen, Xiaoming
中科院分区：
文献类型：
--
作者：
Wang, Haiying;Cui, Kai;Chen, Xiaoming
角瘿因其形状为不规则的菱形而得名，是由食水蚜虫Schlechtendalia chinensis（Bell）攻击盐肤木小枝上的小叶而引起的，其富含没食子单宁，可广泛应用于医药和食品工业。为了解释虫瘿发育的分子机制，我们通过Illumina深度测序和四种组织的数字基因表达进行了转录组分析，包括虫瘿、与虫瘿生长在同一分支上的叶子（GL）、来自没有任何虫瘿的分支的叶子（LW）和来自没有任何虫瘿的树的叶子（CL）。与GL和LW相比，在次生代谢物的生物合成、植物-蚜虫相互作用和植物激素信号转导中丰富的差异表达基因在虫瘿中高度表达。植物激素信号转导以IAA和阿坝为主，协调植物的初生代谢和次生代谢，从而诱导蚜虫为害后虫瘿的诱导和发育。本研究为倍子资源的遗传改良和加工利用提供了理论依据。
Horned gall is named after its shape of irregular diamond, and is induced by the fluid-feeding aphid Schlechtendalia chinensis (Bell) attacks on the leaflets that are located in the branchlets of Rhus chinensis Mill., which is enriched in gallotannin and can be widely used in medicine and the food industry. To explain the molecular mechanism of gall development, we performed transcriptome analysis by Illumina deep sequencing and digital gene expression of four tissues, including galls, leaves that grew on the same branch as the gall taken (GL), leaves from a branch without any gall (LW), and leaves from a tree without any gall (CL). Differentially expressed genes abundantly enriched in the biosynthesis of secondary metabolites, plant-aphid interactions, and plant hormone signal transduction were highly expressed in galls compared with GL and LW. Phytohormone signal transduction, dominated by IAA and ABA, coordinates primary and secondary metabolism and thus induces gall induction and development after attack by aphids. This study provides a theoretical basis for the genetic improvement and processing of gallnut resources.