Mapping neuronal inputs to REM sleep induction sites with carbachol-fluorescent microspheres.
Mapping neuronal inputs to REM sleep induction sites with carbachol-fluorescent microspheres.
复制标题
使用卡巴胆碱荧光微球将神经元输入映射到快速眼动睡眠诱导位点。
DOI：
10.1126/science.2475910
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hobson,JA
中科院分区：
文献类型：
--
作者：
Quattrochi,JJ;Mamelak,AN;Madison,RD;Macklis,JD;Hobson,JA
将胆碱能激动剂卡巴胆碱与用罗丹明荧光标记的乳胶微球缀合，并用作神经解剖学探针，其显示从其注射部位的扩散很少，并逆行标记投射到注射部位的神经元。将这种活性探针显微注射到猫脑桥脑干的神经细胞区域，使清醒的猫陷入快速眼动（REM）睡眠，与游离卡巴胆碱产生的睡眠难以区分。计算机三维重建的逆行标记的神经元显示了广泛分布的脑桥被盖神经元网络。这些微球允许一个新的精度与行为状态和其他胆碱能网络的控制相关的神经元的表征和映射。
The cholinergic agonist carbachol was conjugated to latex microspheres that were fluorescently labeled with rhodamine and used as neuroanatomical probes that show little diffusion from their injection site and retrogradely label neurons projecting to the injection site. Microinjection of this pharmacologically active probe into the gigantocellular field of the cat pontine brain stem caused the awake cats to fall into rapid eye movement (REM) sleep indistinguishable from that produced by free carbachol. Three-dimensional computer reconstruction of the retrogradely labeled neurons revealed a widely distributed neuronal network in the pontine tegmentum. These pharmacologically active microspheres permit a new precision in the characterization and mapping of neurons associated with the control of behavioral state and of other cholinergic networks.