Possible role of interleukin 1 alpha and interleukin 1 beta in the pathogenesis of cholesteatoma of the middle ear.
Possible role of interleukin 1 alpha and interleukin 1 beta in the pathogenesis of cholesteatoma of the middle ear.
复制标题
白细胞介素 1 α 和白细胞介素 1 β 在中耳胆脂瘤发病机制中的可能作用。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
E. Kastenbauer
中科院分区：
文献类型：
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作者：
V. Schilling;B. Negri;J. Bujía;P. Schulz;E. Kastenbauer
中耳胆脂瘤的特征是中耳腔中存在过度增殖的角化鳞状上皮和邻近骨的破坏。白细胞介素1（IL-1）是正常角质形成细胞的自分泌生长因子，并且能够诱导骨降解。用免疫组化法研究了两种IL-1分子，IL-1 α和IL-1 β在中耳炎过度增殖上皮、耳道和耳后区正常表皮以及非角化扁桃体上皮中的分布。在检查的所有鳞状上皮中，IL-1 α和IL-1 β的含量相当。与正常皮肤角质形成细胞相比，中耳炎瘤上皮细胞中IL-1含量明显增加。所有的中耳炎上皮细胞层都对IL-1 α和IL-1 β进行了强烈而均匀的染色，而角蛋白层对IL-1呈阴性。未检测到与基底细胞的特别强的反应。在中耳瘤鳞状上皮下的结缔组织中，强阳性细胞散在分布于阴性基质细胞之间。我们的研究结果表明，IL-1可以从崩解的角质形成细胞和单核细胞-巨噬细胞系的细胞中释放出来，以自分泌的方式刺激中耳炎上皮细胞的增殖，并有助于增强中耳炎存在下的骨破坏。
Cholesteatoma of the middle ear is characterized by the presence of hyperproliferative keratinizing squamous epithelium in the middle ear cavity and destruction of adjacent bone. Interleukin 1 (IL-1) is an autocrine growth factor for normal keratinocytes and is capable of inducing bone degradation. The distribution of two molecular species of IL-1, IL-1 alpha and IL-1 beta, was investigated immunohistochemically in the hyperproliferative epithelium of cholesteatoma, in normal epidermis of the auditory canal and of the retroauricular region, and in nonkeratinizing tonsillar epithelium. In all squamous epithelia examined, IL-1 alpha and IL-1 beta were present in comparable amounts. The IL-1 content of cholesteatoma epithelium was clearly increased in relation to normal skin keratinocytes. All cellular layers of cholesteatoma epithelium stained strongly and uniformly for Il-1 alpha and IL-1 beta, whereas the keratin layer was negative for IL-1. No particularly strong reaction with basal cells was detected. In the connective tissue under the squamous epithelium of cholesteatoma, intensely positive cells were scattered between negative stromal cells. Our results suggest that IL-1 could be liberated from disintegrating keratinocytes and cells of the monocyte-macrophage lineage, stimulate the proliferation of the cholesteatoma epithelium in an autocrine manner, and contribute to the enhancement of bone destruction in the presence of cholesteatoma.