Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARγ2 and C/EBPα in 3T3-L1 cells
Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARγ2 and C/EBPα in 3T3-L1 cells
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DOI：
10.1271/bbb.68.2353
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发表时间：
2004-11-01
影响因子：
1.6
通讯作者：
Ashida, H
中科院分区：
文献类型：
--
作者：
Furuyashiki, T;Nagayasu, H;Ashida, H
肥胖是一个严重的健康问题，通过饮食和锻炼等生活方式来预防肥胖。在本研究中，我们研究了茶儿茶素对3T3-L1前脂肪细胞向脂肪细胞分化的抑制作用。（-）-儿茶素3-没食子儿茶素（CG）、(-)-没食子儿茶素（EGC）、(-)-表儿茶素3-没食子儿茶素3-没食子酸酯和(-)-没食子儿茶素3-没食子酸酯在5mum时抑制细胞内脂质。积累。CG和EGC的抑制作用较强，而且CG和EGC还能抑制作为分化标志的甘油-3-磷酸脱氢酶的活性。这些儿茶素抑制过氧化物酶体增殖激活受体(PPAR) γ ma2和CCAAT/增强子结合蛋白(C/EBP) α的表达，这两种蛋白在分化早期是关键的转录因子，随后在后期是葡萄糖转运蛋白（GLUT） 4的表达。此外，儿茶素不影响胰岛素信号通路的磷酸化状态。因此，儿茶素抑制脂肪细胞分化，同时下调PPARgamma2、C/EBPalpha和GLUT4。这些结果表明，茶儿茶素通过抑制脂肪细胞分化来预防肥胖。
Obesity is a serious health problem, and its prevention is promoted through life style including diet and exercise. In this study, we investigated the suppressive effects of tea catechin on the differentiation of 3T3-L1 preadipocytes to adipocytes. (-)-Catechin 3-gallate (CG), (-)-epigallocatechin (EGC), (-)-epicatechin 3-gallate, and (-)-epigallocatechin 3-gallate at 5 mum suppressed intracellular lipid. accumulation. The suppressive effects of CG and EGC were stronger than the others, and CG and EGC also suppressed the activity of glycerol-3-phosphate dehydrogenase as a differentiation marker. These catechins inhibited the expression of peroxisome proliferator-activated receptor (PPAR) gamma2 and CCAAT/enhancer-binding protein (C/EBP) alpha, both of which act as key transcription factors at an early stage of differentiation, followed by the expression of glucose transporter (GLUT) 4 at a later stage. In addition, the catechins did not affect the phosphorylation status of the insulin signal pathway. Thus, catechin suppressed adipocyte differentiation accompanied by the down-regulation of PPARgamma2, C/EBPalpha, and GLUT4. These results suggest that tea catechin prevents obesity through the suppression of adipocyte differentiation.