Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio)
Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio)
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DOI：
10.1007/s10695-015-0045-x
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发表时间：
2015-03
影响因子：
2.9
通讯作者：
Juan Tian;G. He;K. Mai;Chengdong Liu
中科院分区：
文献类型：
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作者：
Juan Tian;G. He;K. Mai;Chengdong Liu
本研究的目的是系统评估 35 日龄混性斑马鱼 (Danio rerio) 摄食后内分泌、氨基酸和肽转运蛋白以及代谢酶相关基因的分子活性。初始体重为 9 至 11 毫克的斑马鱼在受控的室内环境中禁食 384 小时。在喂食后 0、3、6、12、24、48、96、192 和 384 小时对鱼进行取样。总体而言，本研究结果表明，胰岛素样生长因子I负反馈调节生长激素的调节机制在斑马鱼中与哺乳动物中一样保守，但生长激素受体的调节非常复杂。瘦素和胆囊收缩素是时间依赖性负反馈信号，神经肽Y可能是斑马鱼食物摄入的重要正神经肽。我们的研究中测量的氨基酸/肉碱转运蛋白 B0,+(ATB0,+) 和广泛中性 (0) 氨基酸转运蛋白 1 (B0AT1) mRNA 水平表明，斑马鱼禁食 24-96 小时期间可能会利用蛋白质。本研究中长期禁食后谷氨酰胺合成酶 mRNA 水平下调，谷氨酸脱氢酶、丙氨酸转氨酶、天冬氨酸转氨酶和胰蛋白酶 mRNA 水平上调。禁食96小时后，脂肪酸合成酶的mRNA表达水平显着下降（P<0.05），而脂蛋白脂肪酶的mRNA表达水平迅速上升。短时间禁食会激活葡萄糖合成基因的表达；当禁食时间延长时，葡萄糖分解酶和磷酸戊糖旁路基因的mRNA水平下降。
The goal of this study was to systematically evaluate the molecular activities of endocrine-, amino acid and peptide transporters-, and metabolic enzyme-related genes in 35-day-old mixed-sex zebrafish (Danio rerio) after feeding . Zebrafish with initial body weights ranging from 9 to 11 mg were fasted for 384 h in a controlled indoor environment. Fish were sampled at 0, 3, 6, 12, 24, 48, 96, 192, and 384 h after fed. Overall, the present study results show that the regulatory mechanism that insulin-like growth factor I negative feedback regulated growth hormone is conserved in zebrafish, as it is in mammals, but that regulation of growth hormone receptors is highly intricate. Leptin and cholecystokinin are time-dependent negative feedback signals, and neuropeptide Y may be an important positive neuropeptide for food intake in zebrafish. The amino acid/carnitine transporters B0,+(ATB0,+) and broad neutral (0) amino acid transporter 1(B0AT1) mRNA levels measured in our study suggest that protein may be utilized during 24–96 h of fasting in zebrafish. Glutamine synthetase mRNA levels were downregulated, and glutamate dehydrogenase, alanine aminotransferase, aspartate transaminase, and trypsin mRNA levels were upregulated after longtime fasting in this study. The mRNA expression levels of fatty acid synthetase decreased significantly (P< 0.05), whereas those of lipoprotein lipase rapidly increased after 96 h of fasting. Fasting activated the expression of glucose synthesis genes when fasting for short periods of time; when fasting is prolonged, the mRNA levels of glucose breakdown enzymes and pentose phosphate shunt genes decreased.