Detection of hypothalamic activation by manganese ion contrasted T1-weighted magnetic resonance imaging in rats
Detection of hypothalamic activation by manganese ion contrasted T1-weighted magnetic resonance imaging in rats
复制标题
锰离子对比T1加权磁共振成像检测大鼠下丘脑激活
DOI：
10.1016/s0304-3940(02)00330-0
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发表时间：
2002
影响因子：
2.5
通讯作者：
M. Murakami
中科院分区：
文献类型：
--
作者：
H. Morita;T. Ogino;Y. Seo;N. Fujiki;Kunihiko Tanaka;A. Takamata;Shun;M. Murakami
为了检查Mn 2+对比磁共振成像（MRI）在下丘脑成像中的有用性，将使用T1加权MRI获得的图像与Fos表达进行比较，已知Fos表达在电压依赖性Ca 2+通道激活后增加。静脉输注MnCl 2引起血管和脑室T1加权MRI信号强度迅速增加，但脑实质中没有，表明Mn 2+不能自由扩散穿过血脑屏障。当通过右颈动脉内注射25%d-甘露醇破坏血脑屏障时，在右脑中观察到信号强度增加。颈动脉内注射高渗氯化钠引起室旁核、视上核和视前区信号强度的迅速和显著增加，这些区域被认为参与中枢渗透调节。这些观察结果与Fos表达结果一致。这些结果表明Mn ~（2+）增强MRI是研究下丘脑自主神经中枢的一种有用的技术。
To examine the usefulness of Mn2+contrasted magnetic resonance imaging (MRI) in hypothalamic imaging, images obtained using T1-weighted MRI were compared with Fos expression, which is known to increase after activation of voltage-dependent Ca2+channels. Intravenous infusion of MnCl2elicited a rapid increase in the T1-weighted MRI signal intensity in the vessels and ventricles, but not in the brain parenchyma, suggesting that Mn2+did not diffuse freely across the blood–brain barrier. When the blood–brain barrier was broken by right intracarotid arterial injection of 25% d-mannitol, an increased signal intensity was seen in the right brain. Intracarotid arterial injection of hypertonic NaCl elicited rapid and striking increases in signal intensity in the paraventricular hypothalamic nucleus, supraoptic nucleus, and preoptic area, which are thought to be involved in central osmotic regulation. These observations were consistent with the Fos expression results. These results indicate that Mn2+contrasted MRI is a useful technique for investigating the autonomic center in the hypothalamus.