Anti-biofilm Effect of Dental Adhesive with Cationic Monomer
Anti-biofilm Effect of Dental Adhesive with Cationic Monomer
复制标题
阳离子单体牙科粘合剂的抗生物膜作用
DOI：
10.1177/0022034509334499
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发表时间：
2009-04-01
影响因子：
7.6
通讯作者：
Chen, J. H.
中科院分区：
文献类型：
--
作者：
Li, F.;Chai, Z. G.;Chen, J. H.
加入可聚合的阳离子单体已被尝试合成具有抗菌活性的牙科树脂材料。本研究验证了含有阳离子单体甲基丙烯氧乙基十六烷基二甲基氯化铵(DMAE-CB)的牙科粘合剂对变形链球菌生物膜形成和GTF基因表达的影响。用分光光度法和扫描电子显微镜研究了光聚合DMAE-CB复合胶粘剂对体外生物被膜积累的影响。用实时定量逆转录聚合酶链式反应检测变形链球菌在生物膜中GTF基因的相对表达水平。DMAE-CB复合胶粘剂显著减少了生物膜在其表面的堆积(P<；0.05)，并抑制了变形链球菌在生物膜中的gtfb和gtfc的表达(P<；0.05)。结果表明，固化的DMAE-CB粘合剂可能通过选择性下调GTF基因在变形链球菌中的表达来阻碍生物膜的积累。
The incorporation of polymerizable cationic monomers has been attempted to generate dental resinous materials with antibacterial activity. This study tested the hypothesis that a dental adhesive containing a cationic monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), would influence biofilm formation and gtf gene expression of Streptococcus mutans. The effect of the photo-polymerized DMAE-CB-incorporated adhesive on in vitro biofilm accumulation was investigated with spectrophotometry and scanning electron microscopy. The relative level of gtf gene expression by Streptococcus mutans in the biofilm was quantified by real-time reverse-transcription polymerase chain-reaction. The DMAE-CB-incorporated adhesive significantly decreased biofilm accumulation on its surface (P < 0.05), and suppressed the expression of gtfB and gtfC of Streptococcus mutans in the biofilm (P < 0.05). The results suggest that the cured DMAE-CB-incorporated adhesive may hamper biofilm accumulation via selective down-regulation of the expression of gtf genes in Streptococcus mutans.