Influence of drug treatment on the microacidity in rat and human skin -: An in vitro electron spin resonance imaging study
Influence of drug treatment on the microacidity in rat and human skin -: An in vitro electron spin resonance imaging study
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DOI：
10.1023/a:1011066613621
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发表时间：
2001-04-01
影响因子：
3.7
通讯作者：
Mäder, K
中科院分区：
文献类型：
--
作者：
Kroll, C;Herrmann, W;Mäder, K
目的：探讨体外不同皮肤深度无创检测微酸度(5)的可能性，并研究药物治疗对皮肤内pH值的影响。利用光谱空间电子自旋共振成像（ss-ESRI）和pH敏感的氮氧化物获得了大鼠和人皮肤的体外pH图谱。在皮肤上使用治疗用的酸，如水杨酸和壬二酸，会引起皮肤内微酸度（pH）的明显变化。发现了大鼠和人类皮肤样品在渗透和微酸度方面的物种相关差异。ESRI已被证明是一种新的、完全无创的方法来监测不同皮肤层和皮肤表面的微酸度。这种非破坏性的方法允许在没有任何准备步骤的情况下对皮肤样品进行连续测量。
Purpose, The possibilities of the noninvasive examination of microacidity(5) in different depths of the skin in vitro was explored, and the impact of drug treatment on the pH inside the skin was studied.Methods. Spectral-spatial electron spin resonance imaging (ss-ESRI) and pH-sensitive nitroxides were used to obtain a pH map of rat and human skin in vitro.Results. The dermal application of therapeutically used acids, such as salicylic acid and azelaic acid, caused a plain change of microacidity (pH) inside the skin. Species-linked differences between rat and human skin samples with respect to penetration and microacidity were found.Conclusions. ESRI has been shown to be a new and completely noninvasive method to monitor microacidity in different skin layers and on the skin surface. This nondestructive method allows serial measurements on skin samples to be performed without any preparatory steps.