Interaction between nitric oxide and ethylene in the induction of alternative oxidase in ozone-treated tobacco plants
Interaction between nitric oxide and ethylene in the induction of alternative oxidase in ozone-treated tobacco plants
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DOI：
10.1104/pp.106.085472
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发表时间：
2006-10-01
期刊：
影响因子：
7.4
通讯作者：
Pasqualini, Stefania
中科院分区：
文献类型：
--
作者：
Ederli, Luisa;Morettini, Roberta;Pasqualini, Stefania
高等植物线粒体电子传递链除了细胞色素链外，还包含一条以单一同源二聚体蛋白终止的替代途径，即交替氧化酶(AOX)。我们记录了臭氧(O-3)熏蒸烟草的细胞色素能力、呼吸作用和AOX途径能力的暂时抑制。用AOX1a基因作为分子探针，研究了过氧化氢、一氧化氮(NO)、乙烯(ET)、水杨酸和茉莉酸(JA)等信号分子对AOX1a基因的调控作用，这些分子都被认为参与了O-3反应。熏蒸导致线粒体中过氧化氢的积累和叶片组织中NO的早期积累。虽然在O-3熏蒸开始后5h，叶片组织中的ET积累量较高，但在恢复期，ET积累量有所下降。处理和未处理植株的茉莉酸和12-氧代植物二烯酸水平没有差异。NO、JA和ET诱导AOX1a基因表达增加。通过对ET和NO的药理抑制，我们证明了NO和ET依赖的途径都是O-3诱导AOX1a上调所必需的。然而，只有NO是激活AOX1a基因表达所必需的。
The higher plant mitochondrial electron transport chain contains, in addition to the cytochrome chain, an alternative pathway that terminates with a single homodimeric protein, the alternative oxidase (AOX). We recorded temporary inhibition of cytochrome capacity respiration and activation of AOX pathway capacity in tobacco plants (Nicotiana tabacum L. cv BelW3) fumigated with ozone (O-3). The AOX1a gene was used as a molecular probe to investigate its regulation by signal molecules such as hydrogen peroxide, nitric oxide (NO), ethylene (ET), salicylic acid, and jasmonic acid (JA), all of them reported to be involved in the O-3 response. Fumigation leads to accumulation of hydrogen peroxide in mitochondria and early accumulation of NO in leaf tissues. Although ET accumulation was high in leaf tissues 5 h after the start of O-3 fumigation, it declined during the recovery period. There were no differences in the JA and 12-oxo-phytodienoic acid levels of treated and untreated plants. NO, JA, and ET induced AOX1a mRNA accumulation. Using pharmacological inhibition of ET and NO, we demonstrate that both NO- and ET-dependent pathways are required for O-3-induced up-regulation of AOX1a. However, only NO is indispensable for the activation of AOX1a gene expression.