THE HUMAN MAST-CELL CHYMASE GENE (CMA1) - MAPPING TO THE CATHEPSIN-G GRANZYME GENE-CLUSTER AND LINEAGE-RESTRICTED EXPRESSION
THE HUMAN MAST-CELL CHYMASE GENE (CMA1) - MAPPING TO THE CATHEPSIN-G GRANZYME GENE-CLUSTER AND LINEAGE-RESTRICTED EXPRESSION
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DOI：
10.1006/geno.1993.1115
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发表时间：
1993-03-01
期刊：
影响因子：
4.4
通讯作者：
LEY, TJ
中科院分区：
文献类型：
--
作者：
CAUGHEY, GH;SCHAUMBERG, TH;LEY, TJ
编码T细胞受体α/δ链、中性粒细胞组织蛋白酶G和淋巴细胞CGL/颗粒酶的基因在染色体带14q11.2上紧密连锁。目前的工作确定的人肥大细胞糜蛋白酶基因（CMA 1）作为第四个蛋白酶在这个集群和地图的基因在150 kb的组织蛋白酶G基因。基因顺序为着丝粒-T细胞受体α/δ-CGL-1/颗粒酶B-CGL-2/颗粒酶H-组织蛋白酶G-糜蛋白酶。糜蛋白酶和组织蛋白酶G基因在人肥大细胞系HMC-1和U-937细胞中共转录。其他细胞转录组织蛋白酶G或CGL/颗粒酶基因，但不糜蛋白酶基因，这表明独立调节的能力。糜蛋白酶基因5′侧翼与组织蛋白酶G和CGL/颗粒酶基因的比较显示，总体同源性很小。迄今为止测序的人和鼠糜酶基因只有5′侧翼的短区域相似，表明它们比人和啮齿动物CGL-1/颗粒酶B更远，其侧翼高度同源。基因的表达模式和聚类为基因座控制区的存在提供了可能的线索，该基因座控制区协调白细胞和肥大细胞蛋白酶的谱系限制性表达。
Genes encoding T-cell-receptor α/δ chains, neutrophil cathepsin G, and lymphocyte CGL/granzymes are closely linked on chromosomal band 14q11.2. The current work identifies the human mast cell chymase gene (CMA1) as the fourth protease in this cluster and maps the gene to within 150 kb of the cathepsin G gene. The gene order is centromere-T cell receptor α/δ-CGL-1/granzyme B-CGL-2/granzyme H-cathepsin G-chymase. Chymase and cathepsin G genes are shown to be cotranscribed in the human mast cell line HMC-1 and in U-937 cells. Other cells transcribe cathepsin G or CGL/granzyme genes, but not chymase genes, suggesting a capacity for independent regulation. Comparison of the 5′ flank of the chymase gene with those of cathepsin G and CGL/granzymes reveals little overall homology. Only short regions of the 5′ flanks of the human and murine chymase genes sequenced to date are similar, suggesting that they are more distantly related than human and rodent CGL-1/granzyme B, the flanks of which are highly homologous. The expression patterns and clustering of genes provide possible clues to the presence of locus control regions that orchestrate lineage-restricted expression of leukocyte and mast cell proteases.