Characterization of Mycobacterium avium clinical isolates in Japan using subspecies-specific insertion sequences, and identification of a new insertion sequence, ISMav6
Characterization of Mycobacterium avium clinical isolates in Japan using subspecies-specific insertion sequences, and identification of a new insertion sequence, ISMav6
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DOI：
10.1099/jmm.0.008623-0
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发表时间：
2009-07-01
影响因子：
3
通讯作者：
Ogawa, Kenji
中科院分区：
文献类型：
--
作者：
Ichikawa, Kazuya;Yagi, Tetsuya;Ogawa, Kenji
对81例HIV阴性肺部感染患者和33例HIV阳性肺部感染患者的鸟分枝杆菌临床分离株进行了3种M.禽特异性插入序列（IS 901、IS 1245和IS 1311），以及热休克蛋白65（hsp 65）基因的核苷酸测序。所有临床分离株均鉴定为W. hsp 65的序列分析。与临床分离的M.日本分离株中IS 1245的检出率较低（96/114株，84%），而IS 901的检出率较高（76/114株，67%）。发现一个分离物缺乏IS 1311，这在以前的W.鸟亚种人类的。对IS 901的PCR产物的核苷酸序列分析显示，所有临床分离株具有相同的新插入序列，命名为lSMav 6，与原始IS 901的核苷酸序列相比，其具有60个点突变。这些结果表明V avium subsp.带有ISMav 6的hominissuis在日本很流行。ISMav 6可能与M. avium，并有助于M.禽流感在这个国家。
Clinical isolates of Mycobacterium avium (n=81) from patients with pulmonary infections who were HIV-negative and isolates (n=33) from HIV-positive patients were subjected to genetic analysis by PCR detection of three M. avium-specific insertion sequences (IS901, IS 1245 and IS 1311), and nucleotide sequencing of the heat-shock protein 65 (hsp65) gene. All clinical isolates were identified as W. avium subspecies hominissuis' by sequence analysis of hsp65. Compared with clinical isolates of M. avium reported elsewhere, IS 1245 was found less frequently in Japanese isolates (96/114 isolates, 84%) and IS901 was detected more frequently (76/114 isolates, 67%). One isolate was found to lack IS 1311, which has not been reported previously for W. avium subsp. hominissuis'. Nucleotide sequence analysis of the PCR products for IS901 revealed that all clinical isolates had the same new insertion sequence, designated lSMav6, which had 60 point mutations compared with the nucleotide sequence of the original IS901. These results suggest that V avium subsp. hominissuis with ISMav6 is prevalent in Japan. ISMav6 may have implications for the virulence of M. avium and contribute to an increase of M. avium infections in this country.