Screening applications in drug discovery based on microfluidic technology
Screening applications in drug discovery based on microfluidic technology
复制标题
DOI：
10.1063/1.4940886
复制
发表时间：
2016-01-01
期刊：
影响因子：
3.2
通讯作者：
Ulgen, K. O.
中科院分区：
文献类型：
--
作者：
Eribol, P.;Uguz, A. K.;Ulgen, K. O.
在过去的二十年里，微流控技术因其所有的优点而成为人们关注的焦点，如低化学消耗、减少分析时间、高通量、更好地控制质量和热传递、将台式实验室缩小到芯片，即芯片实验室，以及它提供的许多其他优点。微流控技术在制药行业迅速找到了应用，这需要与尖端科技突破合作，因为药物筛选和商业化是非常漫长和昂贵的过程，而且由于结果不可预测，需要进行许多测试。本文综述了利用微流控技术筛选候选药物的方法，重点介绍了微芯片的制备技术和材料、连续或离散的流动类型及其优点、动力学参数的测定及其与常规系统的比较、毒性和细胞毒性的评估、高通量的浓度生成以及所采用的计算方法。这项审查的一个重要结论是，尽管微流控技术在这一领域已有约20年的历史，但仍有研究和开发的空间，因为这项尖端技术需要独创性来为每个病例设计和找到解决方案。这些微系统的最新扩展是微工程芯片器官和器官阵列。(C)2016 AIP出版有限责任公司。
Microfluidics has been the focus of interest for the last two decades for all the advantages such as low chemical consumption, reduced analysis time, high throughput, better control of mass and heat transfer, downsizing a bench-top laboratory to a chip, i.e., lab-on-a-chip, and many others it has offered. Microfluidic technology quickly found applications in the pharmaceutical industry, which demands working with leading edge scientific and technological breakthroughs, as drug screening and commercialization are very long and expensive processes and require many tests due to unpredictable results. This review paper is on drug candidate screening methods with microfluidic technology and focuses specifically on fabrication techniques and materials for the microchip, types of flow such as continuous or discrete and their advantages, determination of kinetic parameters and their comparison with conventional systems, assessment of toxicities and cytotoxicities, concentration generations for high throughput, and the computational methods that were employed. An important conclusion of this review is that even though microfluidic technology has been in this field for around 20 years there is still room for research and development, as this cutting edge technology requires ingenuity to design and find solutions for each individual case. Recent extensions of these microsystems are microengineered organs-on-chips and organ arrays. (C) 2016 AIP Publishing LLC.