Circadian rhythm of melatonin, corticosterone and phagocytosis:: effect of stress
Circadian rhythm of melatonin, corticosterone and phagocytosis:: effect of stress
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DOI：
10.1034/j.1600-079x.2001.300307.x
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发表时间：
2001-04-01
影响因子：
10.3
通讯作者：
Rodríguez, AB
中科院分区：
文献类型：
--
作者：
Barriga, C;Martín, MI;Rodríguez, AB
褪黑素与免疫系统有功能联系。通过去除松果体（褪黑激素的一个来源），或通过体外培养具有药理浓度的褪黑激素的吞噬细胞，可以改变吞噬细胞的功能。鉴于松果体细胞的合成受交感神经节后神经末梢释放的去甲肾上腺素的控制，本研究旨在评估BALB/c小鼠在基础和应激情况下褪黑激素、皮质酮和吞噬的昼夜节律。以腹膜巨噬细胞作为吞噬细胞，乳胶珠作为摄取颗粒，强迫游泳至精疲力竭作为应激情境。用放射免疫法测定动物血清激素水平。在04:00 - 22:00期间每3小时取样一次，在22:00 - 04:00期间每30分钟取样一次。对照组小鼠的褪黑激素短期峰值出现在33:30小时，而腹腔巨噬细胞的最大惰性颗粒摄取能力也出现在夜间，但在03:30小时。对照组小鼠皮质酮水平呈昼夜节律，在白天（16:00小时）出现峰值。与对照组相比，应激动物的褪黑激素虽然维持在较低的水平，但在23:30小时达到峰值，但血清皮质酮水平的节律丧失，皮质酮水平和巨噬细胞吞噬能力在一天中的任何时间都更大。
Melatonin has a functional connection with the immune system. Phagocyte function is altered by extirpation of the pineal gland, one source of melatonin, or by in vitro incubation of phagocytes with pharmacological concentrations of melatonin. Given that its synthesis by pinealocytes is under the control of the noradrenaline released by the sympathetic postganglionaric nerve endings, the present work was aimed at evaluating the circadian rhythm of melatonin, corticosterone, and phagocytosis in BALB/c mice in basal and stress situations. Peritoneal macrophages were used as phagocytes, latex beads as the particles to be ingested, and forced swimming to exhaustion as the stress situation. Radioimmunoassay was used to determine the animals ' serum hormone levels. Samples were taken every 3 hr in the period from 04:00 to 22:00 hr, and every 30 min during the remaining period from 22:00 to 04:00 hr. Control mice presented a short-term melatonin peak at 33:30 hr, while the maximum inert-particle ingestion capacity of the peritoneal macrophages also occurred during the night but at 03:30 hr. The corticosterone levels in control mice presented a circadian rhythm with a day-time maximum peak (16:00 hr). Compared with the controls, the animals subjected to stress maintained, although at lower values, the melatonin peak at 23:30 hr, but they presented a loss of the rhythm of serum corticosterone levels, and the corticosterone levels and the macrophage phagocytic capacity were greater at all hours of the day.