Glucokinase Regulatory Protein (GCKR) Gene rs4425043 Polymorphism is Associated with Overweight and Obesity in Chinese Women
Glucokinase Regulatory Protein (GCKR) Gene rs4425043 Polymorphism is Associated with Overweight and Obesity in Chinese Women
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葡萄糖激酶调节蛋白（GCKR）基因rs4425043多态性与中国女性超重和肥胖相关
DOI：
10.1007/s11745-011-3533-5
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发表时间：
2011-04-01
期刊：
影响因子：
1.9
通讯作者：
Jin, Li
中科院分区：
文献类型：
--
作者：
Cao, Feifei;Wang, Xiaofeng;Jin, Li
本研究采用全基因标签-单核苷酸多态性（tSNP）技术，探讨GCKR基因多态性对中国女性超重和肥胖患病率的影响。我们在台州市纵向研究的子队列台州市退休妇女队列中进行了遗传关联研究。我们使用Taqman分析法对2,851名女性受试者的GCKR基因的4个tSNPs（rs 4425043、rs780094、rs 814295和rs 8179206）进行基因分型，并研究其与超重和肥胖的相关性。采用有序Logistic回归模型计算比值比及其95%可信区间（CI）。我们观察到rs 4425043与体重指数定义的超重和肥胖之间存在显著关联。rs 4425043基因A等位基因频率从正常体重组（13.20%）、超重组（15.08%）到肥胖组（17.10%）呈显著增加趋势（P = 0.006）。具有GA或AA基因型的个体显示出31%的超重或肥胖的过度风险（95%CI：1.12-1.52，P = 0.001）。此外，我们观察到与rs780094和rs 814295变异相关的空腹血糖水平显著升高（rs780094 AA、GA和GG基因型分别为5.03、5.09和5.15 mmol/L，rs 814295 AA、GA和GG基因型分别为5.03、5.11和5.20 mmol/L）。总之，GCKR基因的一种新的多态性（rs 4425043）增加了中国女性超重和肥胖的风险。以前的报道，GCKR基因的其他多态性与葡萄糖水平也得到了证实。
The aim of this study was to investigate the effects of GCKR polymorphism on the prevalence of overweight and obesity in Chinese female subjects using a gene-wide tagging- single nucleotide polymorphism (tSNP) strategy. We conducted a genetic association study in the Taizhou Retiree Women Cohort, a sub-cohort of the Taizhou longitudinal study. We genotyped four tSNPs (rs4425043, rs780094, rs814295, and rs8179206) of the GCKR gene using the Taqman assay in 2,851 female subjects and investigated their associations with overweight and obesity. Odds ratios and their 95% confidence intervals (CIs) were derived from ordered logistic regression model. We observed significant association between rs4425043 and body-mass-index-defined overweight and obesity. The frequencies of A allele of the rs4425043 exhibited a significant increasing trend from normal weight (13.20%), overweight (15.08%), to obese subjects (17.10%) (P = 0.006). Individuals with the GA or AA genotypes showed a 31% excessive risk to develop overweight or obesity (95% CI: 1.12-1.52, P = 0.001). In addition, we observed significantly increased levels of fasting plasma glucose associated with variations of both rs780094 and rs814295 (5.03, 5.09, and 5.15 mmol/L for rs780094 AA, GA and GG genotypes, respectively, and 5.03, 5.11, and 5.20 mmol/L for rs814295 AA, GA and GG genotypes, respectively). In conclusion, a novel polymorphism (rs4425043) in the GCKR gene increases the risk of overweight and obesity in Chinese women. Previous report that other polymorphisms in the GCKR gene are associated with glucose levels have also been confirmed.