Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system
Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system
复制标题
DOI：
10.1016/j.diagmicrobio.2012.08.013
复制
发表时间：
2012-12-01
影响因子：
2.9
通讯作者：
Poritz, Mark A.
中科院分区：
文献类型：
--
作者：
Blaschke, Anne J.;Heyrend, Caroline;Poritz, Mark A.
败血症是导致死亡的主要原因。直接从血培养中快速准确地识别病原体和抗菌素耐药性可以改善患者的预后。The FilmArray(R)(FA；爱达荷州理工学院，盐湖城。美国德克萨斯大学)血液培养(BC)小组可以在1小时内从阳性血培养中鉴定出25种病原菌和4种抗生素耐药基因。我们将该小组的开发版本与常规培养和102例成人和儿童菌血症存档血培养的药敏试验进行了比较。在培养鉴定的109株病原菌中，有95%是通过FA鉴定出来的。在111个前瞻性收集的血液培养中，FA确定了该小组涵盖的92种病原体中的84种(91%)。在检测到的25株金黄色葡萄球菌和21种肠球菌中，FA鉴定了所有培养证实的耐甲氧西林金黄色葡萄球菌和耐万古霉素肠球菌。FA BC板是从血培养中快速鉴定病原体和耐药基因的准确方法。(C)2012 Elsevier Inc.保留所有权利。
Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray (R) (FA; Idaho Technology, Salt Lake City. UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture. (C) 2012 Elsevier Inc. All rights reserved.