BIOCHEMICAL EVIDENCE THAT NEW INFLUENZA-VIRUS STRAINS IN NATURE MAY ARISE BY RECOMBINATION (REASSORTMENT)
BIOCHEMICAL EVIDENCE THAT NEW INFLUENZA-VIRUS STRAINS IN NATURE MAY ARISE BY RECOMBINATION (REASSORTMENT)
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DOI：
10.1073/pnas.75.7.3341
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
PALESE, P
中科院分区：
文献类型：
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作者：
DESSELBERGER, U;NAKAJIMA, K;PALESE, P
对自然界分离的2种甲型禽流感病毒（Hav6N2和Hav6Nav4）的寡核苷酸分析显示，相应的M和HA基因具有相同或几乎相同的模式：25个大寡核苷酸中有24个和13个大寡核苷酸中有13个通过二维凝胶分析无法区分。观察到其余基因的寡核苷酸模式存在显著差异。NS基因的11个寡核苷酸点中只有2个，NA/NP基因的27个寡核苷酸点中有10个，P基因的49个寡核苷酸点中有22个在两种菌株之间无法区分。这些病毒中至少有2个基因几乎相同，而其他基因则表现出容易检测到的差异，这一观察结果表明，这两种禽流感毒株通过重组事件相互关联。自然界中的动物可双重感染流感病毒。这两方面的证据都有力地表明，重组至少是自然界中出现新的流感病毒株的一种机制。
Oligonucleotide analysis of 2 avian influenza A viruses (Hav6N2 and Hav6Nav4) isolated in nature showed identical or almost identical patterns for the corresponding M and HA genes: 24 of 25 and 13 of 13 large oligonucleotides were indistinguishable by 2-dimensional gel analysis. Remarkable differences in the oligonucleotide patterns of the remaining genes were observed. Only 2 of 11 oligonucleotide spots of the NS gene, 10 of 27 spots of the NA/NP genes and 22 of 49 spots of the P genes were indistinguishable between the 2 strains. This observation that at least 2 genes of these viruses are virtually identical while others show easily detectable differences indicates that the 2 avian strains are related to each other by a recombinational event. Animals in nature can be doubly infected with influenza viruses. Both lines of evidence strongly suggest that recombination is at least 1 mechanism by which new influenza virus strains emerge in nature.