Time of Feeding Alters Obesity-Associated Parameters and Gut Bacterial Communities, but Not Fungal Populations, in C57BL/6 Male Mice
Time of Feeding Alters Obesity-Associated Parameters and Gut Bacterial Communities, but Not Fungal Populations, in C57BL/6 Male Mice
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DOI：
10.1093/cdn/nzz145
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发表时间：
2020-02-01
影响因子：
4.8
通讯作者：
Pierre, Joseph F.
中科院分区：
文献类型：
--
作者：
van der Merwe, Marie;Sharma, Sunita;Pierre, Joseph F.
背景：禁食和定时喂养策略使肥胖参数正常化，即使在高脂肪饮食摄入下。虽然以前的工作表明，这些饮食策略减少肥胖和改善代谢健康，有限的工作已经检查了肠道微生物community.Objectives：我们确定是否定时喂养修改组成的肠道微生物和mycobiome（酵母菌和真菌）Methods：雄性C57 BL/6小鼠喂高脂饮食（HF）6周。然后将动物随机分配到以下组（n = 8-10/组）：1）HF自由采食; 2）纯化高纤维饮食（丹尼尔禁食，DF）; 3）HF时间限制喂养（TRF）（6 h）; 4）HF隔日禁食（ADF）;或5）80%总热量限制（CR）的HF。8周后，肥胖和肠道参数的特点。我们还研究了肠道微生物组和真菌组的变化之前，期间和之后的饮食interventions.Results：体重增加减少与所有限制饮食组。HF喂养的微生物群显示出较低的α多样性沿着拟杆菌门水平降低和厚壁菌门水平增加。从HF切换到DF的动物表现出细菌分类组成，α-和β-多样性的快速转变，最初类似HF，但在DF喂养4周和8周后是不同的。时间或热量限制的HF喂养组没有显示出在门的水平上的变化，但α多样性增加，与特定的属改变。六周的HF喂养减少了各种真菌种群，特别是链格孢属、曲霉属、枝孢属和篮状菌属，并增加了念珠菌属、有孢汉逊酵母属和库尔茨曼氏菌属。然而，8周的干预并没有改变真菌种群，与最丰富的属念珠菌属，青霉属，Hanseniaspora.Conclusions：这些数据表明，定时喂养协议和饮食组成并没有显着影响肠道真菌群落，尽管诱导可测量的变化，在细菌种群，符合代谢的改善。
Background: Fasting and timed feeding strategies normalize obesity parameters even under high-fat dietary intake. Although previous work demonstrated that these dietary strategies reduce adiposity and improve metabolic health, limited work has examined intestinal microbial communities.Objectives: We determined whether timed feeding modifies the composition of the intestinal microbiome and mycobiome (yeast and fungi).Methods: Male C57BL/6 mice were fed a high-fat diet (HF) for 6 wk. Animals were then randomly assigned to the following groups (n = 8-10/group): 1) HF ad libitum; 2) purified high-fiber diet (Daniel Fast, DF); 3) HF-time-restricted feeding (TRF) (6 h); 4) HF-alternate-day fasting (ADF); or 5) HF at 80% total caloric restriction (CR). After 8 wk, obesity and gut parameters were characterized. We also examined changes to the gut microbiome and mycobiome before, during, and following dietary interventions.Results: Body mass gain was reduced with all restricted dietary groups. HF-fed microbiota displayed lower alpha-diversity along with reduced phylum levels of Bacteroidetes and increased Firmicutes. Animals switched from HF to DF demonstrated a rapid transition in bacterial taxonomic composition, alpha-, and beta-diversity that initially resembled HF, but was distinct after 4 and 8 wk of DF feeding. Time-or calorie-restricted HF-fed groups did not show changes at the phylum level, but alpha-diversity was increased, with specific genera altered. Six weeks of HF feeding reduced various fungal populations, particularly Alternaria, Aspergillus, Cladosporium, and Talaromyces, and increased Candida, Hanseniaspora, and Kurtzmaniella. However, 8 wk of intervention did not change the fungal populations, with the most abundant genera being Candida, Penicillium, and Hanseniaspora.Conclusions: These data suggest that timed-feeding protocols and diet composition do not significantly affect the gut fungal community, despite inducing measurable shifts in the bacterial population that coincide with improvements in metabolism.