Comparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens
Comparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens
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DOI：
10.1128/jcm.38.10.3860-3863.2000
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发表时间：
2000-10-01
影响因子：
9.4
通讯作者：
Fischer, SH
中科院分区：
文献类型：
--
作者：
Fahle, GA;Fischer, SH
我们评估了六种市售的DNA提取试剂盒从加入四种不同标本（支气管肺泡灌洗液、脑脊液、血浆和全血）的巨细胞病毒（CMV）的各种稀释液中回收DNA的能力。评价的试剂盒包括Puregene DNA分离试剂盒（PG）、Generation Capture Column试剂盒、MasterPure DNA纯化试剂盒、IsoQuick核酸提取试剂盒、QIAamp血液试剂盒和NucliSens分离试剂盒（NS）。评价的所有六种试剂盒均有效地从四种标本类型中的每一种中去除PCR抑制剂，并产生一致的阳性结果，低至200 PFU/ml的全CMV加标浓度。然而，NS和PG在最低浓度的加标CMV（4和0.4 PFU/ml）下得到最一致的阳性结果，并且在本评价中，提供了从4种不同加标标本中提取CMV DNA的最灵敏方法。还评估了处理时间和成本。
We evaluated six commercially available DNA extraction kits for their ability to recover DNA from various dilutions of cytomegalovirus (CMV) added to four different specimens: bronchoalveolar lavage, cerebral spinal fluid, plasma, and whole blood. The kits evaluated included the Puregene DNA isolation kit (PG), Generation Capture Column kit, MasterPure DNA purification kit, IsoQuick nucleic acid extraction kit, QIAamp blood kit, and NucliSens isolation kit (NS), All six kits evaluated effectively removed PCR inhibitors from each of the four specimen types and produced consistently positive results down to a spiked concentration of 200 PFU of whole CMV per mi. However, the NS and PG resulted in the most consistently positive results at the lowest concentrations of spiked CMV (4 and 0.4 PFU/ml) and, in this evaluation, offered the most sensitive methods for extracting CMV DNA from the four different spiked specimens. Processing time and cost were also evaluated.