Ultrahigh-speed optical coherence tomography imaging and visualization of the embryonic avian heart using a buffered Fourier Domain Mode Locked laser
Ultrahigh-speed optical coherence tomography imaging and visualization of the embryonic avian heart using a buffered Fourier Domain Mode Locked laser
复制标题
DOI：
10.1364/oe.15.006251
复制
发表时间：
2007-05-14
期刊：
影响因子：
3.8
通讯作者：
Rollins, A. M.
中科院分区：
文献类型：
--
作者：
Jenkins, M. W.;Adler, D. C.;Rollins, A. M.
鸡胚胎心脏是研究心脏发育生物学的重要模型。由于缺乏足够的成像技术，从蠕动的心管发育成四腔心脏的机制在很大程度上是未知的。由于活体胚胎鸟类心脏的体积小、运动速度快，需要一种高空间和时间分辨率的成像系统来研究这些模型。在这里，使用缓冲傅立叶域锁模(FDML)激光器的光学相干层析成像(OCT)系统被应用于以每秒100,000次轴向扫描的超高速非侵入性成像。高扫描速率使得能够获取高时间分辨率的2D数据集(每秒195帧或帧之间的5.12毫秒)和3D数据集(每秒10个体积)。分析了以前的OCT技术无法解析的心脏运动的时空细节。可视化和测量技术的发展是为了非侵入性地观察和量化心脏在短暂的收缩(小于50毫秒)和舒张期内的运动。这标志着首次在没有门控的情况下对预贴膜的胚胎禽心进行了4D成像，并首次以极高的时间分辨率在环路过程中观察到了横断面，从而能够观察到心脏在收缩过程中的形态动力学。(C)2007年美国光学学会。
The embryonic avian heart is an important model for studying cardiac developmental biology. The mechanisms that govern the development of a four-chambered heart from a peristaltic heart tube are largely unknown due in part to a lack of adequate imaging technology. Due to the small size and rapid motion of the living embryonic avian heart, an imaging system with high spatial and temporal resolution is required to study these models. Here, an optical coherence tomography ( OCT) system using a buffered Fourier Domain Mode Locked (FDML) laser is applied for ultrahigh-speed non-invasive imaging of embryonic quail hearts at 100,000 axial scans per second. The high scan rate enables the acquisition of high temporal resolution 2D datasets (195 frames per second or 5.12 ms between frames) and 3D datasets (10 volumes per second). Spatio-temporal details of cardiac motion not resolvable using previous OCT technology are analyzed. Visualization and measurement techniques are developed to non-invasively observe and quantify cardiac motion throughout the brief period of systole ( less than 50 msec) and diastole. This marks the first time that the preseptated embryonic avian heart has been imaged in 4D without the aid of gating and the first time it has been viewed in cross section during looping with extremely high temporal resolution, enabling the observation of morphological dynamics of the beating heart during systole. (C) 2007 Optical Society of America.