Effects of hydrogen peroxide (H2O2) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines
Effects of hydrogen peroxide (H2O2) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines
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DOI：
10.1007/s10565-006-0018-z
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发表时间：
2006-01-01
影响因子：
6.1
通讯作者：
Yang, HC
中科院分区：
文献类型：
--
作者：
Lee, DH;Lim, BS;Yang, HC
过氧化氢(H_2O_2)是一种氧化剂，被广泛用作消毒剂。最近，由于过氧化氢的活性，它也被用作牙齿漂白剂在牙齿护理中。这是一个令人担忧的原因，因为它对口腔环境的软组织和硬组织有不利的生物学影响。为了探讨H_2O_2对牙髓中产生牙本质的成牙本质细胞的影响，我们检测了H_2O_2处理后的成牙本质细胞系(MDPC-23)的细胞活力、活性氧(ROS)生成、碱性磷酸酶(ALP)活性和结节形成情况，并与成骨前细胞MC3T3-E1细胞进行了比较。H_2O_2对MDPC-23细胞和MC3T_3-E_1细胞的细胞毒作用均始于0.3 mmol/L。在该浓度下，荧光探针DCFH-DA证实了细胞内ROS的积累。虽然在MDPC-23细胞中检测到更多的ROS，但两种细胞的ROS增加的方式和速度相似。当H_2O_2浓度低于0.3 mmol/L时，MDPC-23细胞的碱性磷酸酶活性和矿化骨基质显著增加，而MC3T_3-E_1细胞则表现出相反的作用。众所周知，ROS通常是有氧生活的有害副产品，是衰老和许多疾病的主要原因。然而，这些数据表明，ROS可以在体外诱导细胞分化，并且它们在细胞生理学中发挥着比简单地引起氧化损伤更复杂的作用。
Hydrogen peroxide (H2O2), an oxidizing agent, has been widely used as a disinfectant. Recently, because of its reactive properties, H2O2 has also been used as a tooth bleaching agent in dental care. This is a cause for concern because of adverse biological effects on the soft and hard tissues of the oral environment. To investigate the influence of H2O2 on odontoblasts, the cells producing dentin in the pulp, we assessed cellular viability, generation of reactive oxygen species (ROS), alkaline phosphatase (ALP) activity, and nodule formation of an odontoblastic cell line (MDPC-23) after treatment with H2O2, and compared those with the effects on preosteoblastic MC3T3-E1 cells. Cytotoxic effects of H2O2 began to appear at 0.3 mmol/L in both MDPC-23 and MC3T3-E1 cells. At that concentration, the accumulation of intracellular ROS was confirmed by a fluorescent probe, DCFH-DA. Although more ROS were detected in MDPC-23, the increasing pattern and rate are similar between the two cells. When the cells were treated with H2O2 at concentrations below 0.3 mmol/L, MDPC-23 displayed a significant increase in ALP activity and mineralized bone matrix, while MC3T3-E1 cells showed adverse effects of H2O2. It is known that ROS are generally harmful by-products of aerobic life and represent the primary cause of aging and numerous diseases. These data, however, suggest that ROS can induce in vitro cell differentiation, and that they play a more complex role in cell physiology than simply causing oxidative damage.