Effect of Schinus terebinthifolius on Candida albicans growth kinetics, cell wall formation and micromorphology
Effect of Schinus terebinthifolius on Candida albicans growth kinetics, cell wall formation and micromorphology
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DOI：
10.3109/00016357.2012.741694
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发表时间：
2013-05-01
影响因子：
2
通讯作者：
de Castro, Ricardo Dias
中科院分区：
文献类型：
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作者：
Alves, Livia Araujo;Freires, Irlan de Almeida;de Castro, Ricardo Dias
目的：评价巴西胡椒(Schinus Terebinthifolius)酊剂对口腔真菌感染相关微生物白念珠菌(ATCC 289065)的抗真菌活性。材料和方法：采用微量稀释法测定最低抑菌浓度(MIC)和最低杀菌浓度(MFC)，并测定其对白色念珠菌的促生生长曲线。此外，本研究还研究了该产物对真菌细胞壁的可能活性及其对真菌形态的生物活性。以制霉菌素为对照，所有试验一式三份。结果：对供试菌株的最低抑菌浓度和最低抑菌浓度分别为312.5和2500mU g/mL，制霉菌素对受试菌株的最低抑菌浓度和最低抑菌浓度分别为6.25mU g/mL和6.25mU g/mL。在微生物生长曲线方面，直到60min，与生长对照相比，Terebinthifolius能够显著降低CFU/mL的数量。在120min和180min时，生长控制剂和试验品之间差异无统计学意义。一旦山梨醇试验显示最低抑菌浓度为1250微克/毫升，该菌可能作用于真菌细胞壁。在真菌形态上，在实验产物的存在下，观察到假菌丝、衣原体分生孢子和芽孢子的减少。结论：Terebinthifolius对白色念珠菌具有抗真菌活性，可能抑制真菌细胞壁的形成。
Objective: To evaluate the anti-fungal activity of a tincture from Schinus terebinthifolius (Brazilian pepper tree) on Candida albicans (ATCC 289065), a micro-organism associated with fungal infections of the oral cavity. Materials and methods: Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were determined through microdilution technique, as well as the microbial growth curve of C. albicans promoted by S. terebinthifolius. In addition, this study investigated a possible activity of the product on the fungal cell wall and its biological activity on fungal morphology. Nystatin was used as control and all tests were performed in triplicate. Results: S. terebinthifolius showed MIC of 312.5 mu g/mL and MFC of 2500 mu g/mL upon the strain tested, while Nystatin showed MIC andMFC of 6.25 mu g/mL. As regards the microbial growth curve, S. terebinthifolius was able to significantly reduce the number of CFU/mL when compared to growth control until the time of 60 min. In the times 120 and 180 min there was no statistically significant difference between the growth control and the experimental product. S. terebinthifolius possibly acts on the fungal cell wall, once the sorbitol test indicated a MIC of 1250 mu g/mL. In the fungal morphology, a reduction was observed of pseudo-hyphae, chlamydoconidia and blastoconidia in the presence of the experimental product. Conclusion: S. terebinthifolius showed anti-fungal activity against C. albicans, inhibiting, probably, the fungal cell wall formation.