A role for somatomedin C as a differentiating hormone and amplifier of hormone action on ovarian cells: studies with synthetically pure human somatomedin C and swine granulosa cells.
A role for somatomedin C as a differentiating hormone and amplifier of hormone action on ovarian cells: studies with synthetically pure human somatomedin C and swine granulosa cells.
复制标题
生长调节素 C 作为卵巢细胞的分化激素和激素作用放大器的作用：对合成纯人生长调节素 C 和猪颗粒细胞的研究。
DOI：
10.1016/0006-291x(85)90407-3
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Demers,LM
中科院分区：
文献类型：
--
作者：
Veldhuis,JD;Demers,LM
在体外化学培养基中培养的分离猪颗粒细胞对合成的纯人生长激素C IGF-I有剂量和时间依赖性的反应，并增加孕烯醇酮、孕酮和雌二醇的生物合成。这些刺激作用不能被生长激素、胰岛素原、去肽胰岛素、表皮生长因子或成纤维细胞生长因子所模仿。此外，生长激素C IGF-I增强了颗粒细胞对外源低密度脂蛋白形式的固醇底物的类固醇生成反应，并以协同方式放大了经典卵巢效应激素雌二醇和促卵泡激素的刺激作用。一方面，生长激素C IGF-I能够刺激雌二醇的产生，另一方面，与雌二醇协同作用刺激孕酮的生物合成，这表明颗粒细胞中有一种独特的卵泡内机制可以增强孕酮的生物合成能力。
Isolated swine granulosa cells incubated in chemically defined medium in vitro responded to synthetically pure human somatomedin C IGF-I in a dose and time-dependent fashion with increased pregnenolone, progesterone and estradiol biosynthesis. These stimulatory actions were not mimicked by growth hormone, proinsulin, desoctapeptide insulin, epidermal growth factor, or fibroblast growth factor. Moreover, somatomedin C IGF-I augmented the steroidogenic response of granulosa cells to exogenously supplied sterol substrate in the form of low-density lipoprotein, and amplified the stimulatory actions of the classical ovarian effector hormones, estradiol and follicle-stimulating hormone, in a synergistic fashion. The ability of somatomedin C IGF-I to stimulate estradiol production on the one hand, and to act synergistically with estradiol to stimulate progesterone biosynthesis on the other hand, suggests a unique intrafollicular mechanism for amplifying progestin biosynthetic capacity in granulosa cells.