Tissue-specific and dexamethasone-inducible expression of alkaline phosphatase from alternative promoters of the rat bone/liver/kidney/placenta gene.
Tissue-specific and dexamethasone-inducible expression of alkaline phosphatase from alternative promoters of the rat bone/liver/kidney/placenta gene.
复制标题
来自大鼠骨/肝/肾/胎盘基因的替代启动子的碱性磷酸酶的组织特异性和地塞米松诱导表达。
DOI：
10.1016/0006-291x(91)90405-v
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发表时间：
1991
影响因子：
3.1
通讯作者：
Twarog,K
中科院分区：
文献类型：
--
作者：
Zernik,J;Kream,B;Twarog,K
摘要大鼠骨/肝/肾/胎盘碱性磷酸酶（ALP）由两个间隔超过25 kb的启动子转录，导致两个编码序列相同的变体转录本。我们研究了稳态水平的两种变体转录在不同的大鼠组织和细胞系中使用的聚合酶链反应（PCR）扩增的RNA表型，RNA酶保护，和北方印迹分析。我们的研究结果表明，从上游启动子的ALP成绩单优先在头盖骨中表达，几乎只在ROS 17 2.8成骨肉瘤细胞中表达。相反，下游启动子优先在肾脏中表达。此外，地塞米松处理ROS 17 2.8细胞后ALP活性和mRNA水平的增加与上游启动子转录物水平的增加相关。因此，大鼠BLKP ALP基因的两个替代启动子参与其表达的细胞特异性和地塞米松诱导的调节。
Abstract The rat Bone/Liver/Kidney/Placenta Alkaline Phosphatase (ALP) is transcribed from two alternative promoters spaced over 25 kb apart, resulting in two variant transcripts that are identical in their coding sequence. We investigated the steady-state levels of the two variant transcripts in various rat tissues and cell lines using the polymerase chain reaction (PCR) amplification for RNA phenotyping, RNase protection, and northern blot analysis. Our results demonstrate that ALP transcripts from the upstream promoter are preferentially expressed in calvariae, and are almost exclusively expressed in ROS17 2.8 osteogenic sarcoma cells. In contrast, the downstream promoter is preferentially expressed in kidney. Moreover, the increase in ALP activity and mRNA levels following dexamethasone treatment of ROS17 2.8 cells is correlated with an increase in the level of transcripts from the upstream promoter. Thus, the two alternative promoters of the rat BLKP ALP gene are involved in cell-specific and dexamethasone-inducible regulation of its expression.