Breeding of High Cooking and Eating Quality in Rice by Marker-Assisted Backcrossing (MABc) Using KASP Markers.
Breeding of High Cooking and Eating Quality in Rice by Marker-Assisted Backcrossing (MABc) Using KASP Markers.
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DOI：
10.3390/plants10040804
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发表时间：
2021-04-19
期刊：
影响因子：
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通讯作者：
Cho YG
中科院分区：
文献类型：
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作者：
Kim MS;Yang JY;Yu JK;Lee Y;Park YJ;Kang KK;Cho YG
水稻育种计划的主要目标是提高粮食质量和产量潜力。根据人们对优质煮食品质水稻品种的需求，我们通过标记辅助回交（MABc）育种计划，与sam光和Milkyqueen杂交，开发出低直链淀粉含量的选育品系。通过SNP作图活动，鉴定出与低直链淀粉含量相关的siiia基因性状标记，并利用这些标记选择有利的株系进行前景选择。为了快速恢复Samgwang （recurrent parent genome， RPG）的遗传背景，利用386个全基因组标记筛选BC1F1和BC2F1个体。选择7个具有目标性状的BC2F1系，遗传背景恢复范围在RPG的97.4 ~ 99.1%之间。所选BC2F2籽粒直链淀粉含量在12.4 ~ 16.8%之间。我们使用性状和全基因组标记证明了MABc，使我们能够有效地选择目标性状的株系，并有效地缩短育种周期。此外，本研究确定的BC2F2分子标记系可作为后续优质烹饪和食用大米育种的亲本系。
The primary goals of rice breeding programs are grain quality and yield potential improvement. With the high demand for rice varieties of premium cooking and eating quality, we developed low-amylose content breeding lines crossed with Samgwang and Milkyqueen through the marker-assisted backcross (MABc) breeding program. Trait markers of the SSIIIa gene referring to low-amylose content were identified through an SNP mapping activity, and the markers were applied to select favorable lines for a foreground selection. To rapidly recover the genetic background of Samgwang (recurrent parent genome, RPG), 386 genome-wide markers were used to select BC1F1 and BC2F1 individuals. Seven BC2F1 lines with targeted traits were selected, and the genetic background recovery range varied within 97.4–99.1% of RPG. The amylose content of the selected BC2F2 grains ranged from 12.4–16.8%. We demonstrated the MABc using a trait and genome-wide markers, allowing us to efficiently select lines of a target trait and reduce the breeding cycle effectively. In addition, the BC2F2 lines confirmed by molecular markers in this study can be utilized as parental lines for subsequent breeding programs of high-quality rice for cooking and eating.