Parasporin, a new anticancer protein group from Bacillus thuringiensis.
Parasporin, a new anticancer protein group from Bacillus thuringiensis.
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发表时间：
2009
影响因子：
2
通讯作者：
M. Ohba;E. Mizuki;Akiko Uemori
中科院分区：
文献类型：
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作者：
M. Ohba;E. Mizuki;Akiko Uemori
副孢子蛋白（PS）是苏云金芽孢杆菌中合成的一系列系谱异质性Cry蛋白。通常与PS蛋白相关的一个突出特征是对各种来源的人癌细胞优先的强杀细胞活性。这些蛋白质只有在用蛋白酶消化时才表现出杀细胞活性。目前，该蛋白质组被分为四个家族：PS1、PS2、PS3和PS4。四个PS家族之间的细胞毒性谱和活性水平存在明显差异。溶血活性和昆虫毒性都与PS蛋白无关。在PS1Aa1诱导的事件中，最引人注目的方面之一是细胞内Ca 2+浓度的早期和快速增加，而质膜透性没有变化。有强有力的证据表明PS1Aa1通过凋亡杀死癌细胞。与PS1Aa1不同，PS2Aa1增加癌细胞的质膜通透性。PS2Aa1的细胞杀伤作用的初始步骤是这种细胞毒素与位于脂筏中的假定受体的特异性结合，随后是其寡聚化和质膜中的孔形成。
Parasporin (PS) is a collection of genealogically heterogeneous Cry proteins synthesized in Bacillus thuringiensis. A prominent feature commonly associated with PS proteins is the strong cytocidal activity preferential for human cancer cells of various origins. The proteins exhibit cytocidal activities only when digested with proteases. Currently, this protein group is classified into four families: PS1, PS2, PS3 and PS4. Marked differences are evident in cytotoxicity spectra and activity levels between the four PS families. Neither hemolytic activity nor insect toxicity is associated with PS proteins. One of the most striking aspects in the events induced by PS1Aa1 is the early and rapid increase of the intracellular Ca2+ concentration, with no change in plasma membrane permeability. There is strong evidence that PS1Aa1 kills cancer cells through apoptosis. Unlike PS1Aa1, PS2Aa1 increases plasma membrane permeability of cancer cells. The initial step in cytocidal action of PS2Aa1 is the specific binding of this cytotoxin to a putative receptor located in the lipid rafts, followed by its oligomerization and pore formation in plasma membrane.