ELECTRICALLY CHARGED POLYMERIC SUBSTRATES ENHANCE NERVE-FIBER OUTGROWTH INVITRO
ELECTRICALLY CHARGED POLYMERIC SUBSTRATES ENHANCE NERVE-FIBER OUTGROWTH INVITRO
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DOI：
10.1016/0142-9612(92)90069-z
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发表时间：
1992-01-01
期刊：
影响因子：
14
通讯作者：
AEBISCHER, P
中科院分区：
文献类型：
--
作者：
VALENTINI, RF;VARGO, TG;AEBISCHER, P
已知合成引导装置的物理、化学和电学性质影响体内神经再生。在本研究中，将神经元直接培养在带电的聚合物生长基质上，以确定局部电荷是否能增强体外神经纤维的生长。压电聚合物如聚偏二氟乙烯（PVDF）在微小的机械应变下产生瞬态表面电荷。将小鼠神经母细胞瘤（Nb 2a）细胞直接培养在无血清和含血清培养基中的电极化（即压电）和非电极化（即非压电）PVDF基底上。在铺板后24、48、72和96小时分析神经纤维生长。当放置在标准培养箱架上时，压电PVDF基板在1200 Hz下产生2-3 mV，并且未极化的PVDF基板显示无输出。在压电基板上生长的Nb 2a细胞表现出显着更大的水平的过程中生长和神经突长度在所有时间段的两种介质条件。详细的表面表征的PVDF基板，使用电子能谱化学分析（ESCA）和全面的润湿性分布显示，极化和未极化的PVDF是化学上无法区分的，并表现出类似的表面润湿性和粘合性能。因此，我们的结论是，增强的过程生长诱导的薄膜的压电输出，使极化PVDF一种独特的生物材料，细胞/聚合物的相互作用介导的主要是通过体电性能，而不是表面性能。
The physical, chemical and electrical properties of synthetic guidance devices are known to influence nerve regeneration in vivo. In the present study, neurons were cultured directly on electrically charged polymer growth substrates to determine if local electrical charges enhance nerve fibre outgrowth in vitro. Piezoelectric polymers such as polyvinylidene fluoride (PVDF) generate transient surface charges under minute mechanical strain. Mouse neuroblastoma (Nb2a) cells were cultured directly on electrically poled (i.e. piezoelectric) and unpoled (i.e. non-piezoelectric) PVDF substrates in serum-free and serum-containing media. Nerve fibre outgrowth was analysed 24, 48, 72 and 96 h after plating. Piezoelectric PVDF substrates generated 2-3 mV at 1200 Hz when placed on standard incubator shelves and unpoled PVDF substrates showed no output. Nb2a cells grown on piezoelectric substrates exhibited significantly greater levels of process outgrowth and neurite lengths at all time periods for both media conditions. Detailed surface characterization of PVDF substrates using electron spectroscopy for chemical analysis (ESCA) and a comprehensive wettability profile revealed that poled and unpoled PVDF was chemically indistinguishable and showed similar surface wettabilities and adhesive properties. Therefore, we conclude that enhanced process outgrowth was induced by the film's piezoelectric output, making poled PVDF a unique biomaterial for which cell/polymer interactions are mediated predominantly through bulk electrical properties rather than surface properties.