Reclassification of salt-water Bdellovibrio sp as Bacteriovorax marinus sp nov and Bacteriovorax litoralis sp nov.
Reclassification of salt-water Bdellovibrio sp as Bacteriovorax marinus sp nov and Bacteriovorax litoralis sp nov.
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DOI：
10.1099/ijs.0.02458-0
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发表时间：
2004-07-01
影响因子：
2.8
通讯作者：
Williams, HN
中科院分区：
文献类型：
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作者：
Baer, ML;Ravel, J;Williams, HN
蜈蚣弧菌是一种独特的捕食性细菌，在其猎物内有一个周质内的生长和繁殖阶段，由许多革兰氏阴性细菌组成。直到最近，所有表现出这些特征的细菌都被归入蜈蚣弧菌属。然而，对16S rDNA序列的分析和其他研究表明，该属生物之间存在着相当大的遗传型、表型和生态型多样性(Baer等人，2000年；Snyder等人，2002年)。这导致将蜈蚣弧菌和斯塔里青春弧菌重新归类为新构建的杆状杆菌属(Baer等人，2000年)。在这项研究中，对海洋蛭弧菌分离株(命名为SJ(T)，AQ和JS5(T))的检测表明，它们与新命名的杆菌属关系更近。16S rRNA基因序列的系统发育分析表明，海洋分离株SJ(T)、AQ和JS5(T)与细小芽孢杆菌100(T)聚为一个独立的分支，属于细小芽孢杆菌100(T)的一个分支，这表明它们在分类上与后者有较近的亲缘关系。DNA-DNA杂交实验也证明了
Bdellovibrios are unique, predatory bacteria with an intraperiplasmic growth and multiplication phase within their prey, which consists of many Gram-negative bacteria. Until recently, all bacteria that exhibited these traits were included in the genus Bdellovibrio. However, analysis of 16S rDNA sequences and other studies have demonstrated substantial genotypic, phenotypic and ecotypic diversity among the organisms in this genus (Baer et al., 2000; Snyder et al., 2002). This has resulted in reclassification of Bdellovibrio stolpii and Bdellovibrio starrii into the newly constructed genus Bacteriovorax (Baer et al., 2000). In this study, examination of marine isolates of Bdellovibrio (designated SJ(T), AQ and JS5(T)) has revealed them to be related more closely to the newly designated genus Bacteriovorax. Phylogenetic analysis of 16S rRNA gene sequences revealed that marine isolates SJ(T), AQ and JS5(T) clustered in a separate clade from Bdellovibrio bacteriovorus 100(T) as part of the clade that contains Bacteriovorax spp., indicating a much closer taxonomic relationship to the latter. DNA-DNA hybridization experiments also demonstrated